Sökning: onr:"swepub:oai:DiVA.org:su-196789" > MyD88 TIR domain hi...
Fältnamn | Indikatorer | Metadata |
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000 | 03599naa a2200601 4500 | |
001 | oai:DiVA.org:su-196789 | |
003 | SwePub | |
008 | 210929s2021 | |||||||||||000 ||eng| | |
024 | 7 | a https://urn.kb.se/resolve?urn=urn:nbn:se:su:diva-1967892 URI |
024 | 7 | a https://doi.org/10.1038/s41467-021-22590-62 DOI |
040 | a (SwePub)su | |
041 | a engb eng | |
042 | 9 SwePub | |
072 | 7 | a ref2 swepub-contenttype |
072 | 7 | a art2 swepub-publicationtype |
100 | 1 | a Clabbers, Max T. B.u Stockholms universitet,Institutionen för material- och miljökemi (MMK)4 aut0 (Swepub:su)macl1978 |
245 | 1 0 | a MyD88 TIR domain higher-order assembly interactions revealed by microcrystal electron diffraction and serial femtosecond crystallography |
264 | c 2021-05-10 | |
264 | 1 | b Springer Science and Business Media LLC,c 2021 |
338 | a print2 rdacarrier | |
520 | a MyD88 and MAL are Toll-like receptor (TLR) adaptors that signal to induce pro-inflammatory cytokine production. We previously observed that the TIR domain of MAL (MALTIR) forms filaments in vitro and induces formation of crystalline higher-order assemblies of the MyD88 TIR domain (MyD88TIR). These crystals are too small for conventional X-ray crystallography, but are ideally suited to structure determination by microcrystal electron diffraction (MicroED) and serial femtosecond crystallography (SFX). Here, we present MicroED and SFX structures of the MyD88TIR assembly, which reveal a two-stranded higher-order assembly arrangement of TIR domains analogous to that seen previously for MALTIR. We demonstrate via mutagenesis that the MyD88TIR assembly interfaces are critical for TLR4 signaling in vivo, and we show that MAL promotes unidirectional assembly of MyD88TIR. Collectively, our studies provide structural and mechanistic insight into TLR signal transduction and allow a direct comparison of the MicroED and SFX techniques. | |
650 | 7 | a NATURVETENSKAPx Biologi0 (SwePub)1062 hsv//swe |
650 | 7 | a NATURAL SCIENCESx Biological Sciences0 (SwePub)1062 hsv//eng |
700 | 1 | a Holmes, Susannah4 aut |
700 | 1 | a Muusse, Timothy W.4 aut |
700 | 1 | a Vajjhala, Parimala R.4 aut |
700 | 1 | a Thygesen, Sara J.4 aut |
700 | 1 | a Malde, Alpeshkumar K.4 aut |
700 | 1 | a Hunter, Dominic J. B.4 aut |
700 | 1 | a Croll, Tristan4 aut |
700 | 1 | a Flueckiger, Leonie4 aut |
700 | 1 | a Nanson, Jeffrey D.4 aut |
700 | 1 | a Rahaman, Md Habibur4 aut |
700 | 1 | a Aquila, Andrew4 aut |
700 | 1 | a Hunter, Mark S.4 aut |
700 | 1 | a Liang, Mengning4 aut |
700 | 1 | a Yoon, Chun Hong4 aut |
700 | 1 | a Zhao, Jingjingu Stockholms universitet,Institutionen för material- och miljökemi (MMK)4 aut0 (Swepub:su)jinzh |
700 | 1 | a Zatsepin, Nadia A.4 aut |
700 | 1 | a Abbey, Brian4 aut |
700 | 1 | a Sierecki, Emma4 aut |
700 | 1 | a Gambin, Yann4 aut |
700 | 1 | a Stacey, Katryn J.4 aut |
700 | 1 | a Darmanin, Connie4 aut |
700 | 1 | a Kobe, Bostjan4 aut |
700 | 1 | a Xu, Hongyiu Stockholms universitet,Institutionen för material- och miljökemi (MMK)4 aut0 (Swepub:su)hxu |
700 | 1 | a Ve, Thomas4 aut |
710 | 2 | a Stockholms universitetb Institutionen för material- och miljökemi (MMK)4 org |
773 | 0 | t Nature Communicationsd : Springer Science and Business Media LLCg 12:1q 12:1x 2041-1723 |
856 | 4 | u https://doi.org/10.1038/s41467-021-22590-6y Fulltext |
856 | 4 | u https://www.nature.com/articles/s41467-021-22590-6.pdf |
856 | 4 8 | u https://urn.kb.se/resolve?urn=urn:nbn:se:su:diva-196789 |
856 | 4 8 | u https://doi.org/10.1038/s41467-021-22590-6 |
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