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Heimdallarchaea enc...
Heimdallarchaea encodes profilin with eukaryotic-like actin regulation and polyproline binding
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- Survery, Sabeen (författare)
- Stockholms universitet,Institutionen för molekylär biovetenskap, Wenner-Grens institut,Stockholm Univ, Wenner Gren Inst, Dept Mol Biosci, Stockholm, Sweden.
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- Hurtig, Fredrik (författare)
- Stockholms universitet,Institutionen för molekylär biovetenskap, Wenner-Grens institut,Stockholm Univ, Wenner Gren Inst, Dept Mol Biosci, Stockholm, Sweden.
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- Haq, Syed Razaul (författare)
- Stockholms universitet,Institutionen för molekylär biovetenskap, Wenner-Grens institut,Stockholm Univ, Wenner Gren Inst, Dept Mol Biosci, Stockholm, Sweden.
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- Eriksson, Jens, 1982- (författare)
- Uppsala universitet,Institutionen för medicinsk biokemi och mikrobiologi,Infektionsbiologi
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- Guy, Lionel, PhD, Docent, 1980- (författare)
- Uppsala universitet,Institutionen för medicinsk biokemi och mikrobiologi,Infektionsbiologi
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- Rosengren, K. Johan (författare)
- Univ Queensland, Sch Biomed Sci, Brisbane, Qld, Australia.
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- Lindås, Ann-Christin (författare)
- Stockholms universitet,Institutionen för molekylär biovetenskap, Wenner-Grens institut,Stockholm Univ, Wenner Gren Inst, Dept Mol Biosci, Stockholm, Sweden.
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- Chi, Celestine N. (författare)
- Uppsala universitet,Institutionen för medicinsk biokemi och mikrobiologi
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(creator_code:org_t)
- 2021-09-01
- 2021
- Engelska.
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Ingår i: Communications Biology. - : Springer Science and Business Media LLC. - 2399-3642. ; 4:1
- Relaterad länk:
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https://doi.org/10.1...
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https://www.nature.c...
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https://uu.diva-port... (primary) (Raw object)
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https://urn.kb.se/re...
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https://doi.org/10.1...
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https://urn.kb.se/re...
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Abstract
Ämnesord
Stäng
- It is now widely accepted that the first eukaryotic cell emerged from a merger of an archaeal host cell and an alphaproteobacterium. However, the exact sequence of events and the nature of the cellular biology of both partner cells is still contentious. Recently the structures of profilins from some members of the newly discovered Asgard superphylum were determined. In addition, it was found that these profilins inhibit eukaryotic rabbit actin polymerization and that this reaction is regulated by phospholipids. However, the interaction with polyproline repeats which are known to be crucial for the regulation of profilin:actin polymerization was found to be absent for these profilins and was thus suggested to have evolved later in the eukaryotic lineage. Here, we show that Heimdallarchaeota LC3, a candidate phylum within the Asgard superphylum, encodes a putative profilin (heimProfilin) that interacts with PIP2 and its binding is regulated by polyproline motifs, suggesting an origin predating the rise of the eukaryotes. More precisely, we determined the 3D-structure of Heimdallarchaeota LC3 profilin and show that this profilin is able to: i) inhibit eukaryotic actin polymerization in vitro; ii) bind to phospholipids; iii) bind to polyproline repeats from enabled/vasodilator‐stimulated phosphoprotein; iv) inhibit actin from Heimdallarchaeota from polymerizing into filaments. Our results therefore provide hints of the existence of a complex cytoskeleton already in last eukaryotic common ancestor.
Ämnesord
- NATURVETENSKAP -- Biologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences (hsv//eng)
- NATURVETENSKAP -- Biologi -- Cellbiologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Cell Biology (hsv//eng)
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Till lärosätets databas
- Av författaren/redakt...
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Survery, Sabeen
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Hurtig, Fredrik
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Haq, Syed Razaul
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Eriksson, Jens, ...
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Guy, Lionel, PhD ...
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Rosengren, K. Jo ...
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visa fler...
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Lindås, Ann-Chri ...
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Chi, Celestine N ...
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visa färre...
- Om ämnet
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- NATURVETENSKAP
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NATURVETENSKAP
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och Biologi
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- NATURVETENSKAP
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NATURVETENSKAP
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och Biologi
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och Cellbiologi
- Artiklar i publikationen
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Communications B ...
- Av lärosätet
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Stockholms universitet
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Uppsala universitet