Sökning: onr:"swepub:oai:DiVA.org:su-20346" >
Correlation of sist...
-
Matsuoka, Atsuko
(författare)
Correlation of sister chromatid exchange formation through homologous recombination with ribonucleotide reductase inhibition.
- Artikel/kapitelEngelska2004
Förlag, utgivningsår, omfång ...
Nummerbeteckningar
-
LIBRIS-ID:oai:DiVA.org:su-20346
-
https://urn.kb.se/resolve?urn=urn:nbn:se:su:diva-20346URI
Kompletterande språkuppgifter
-
Språk:engelska
-
Sammanfattning på:engelska
Ingår i deldatabas
Klassifikation
-
Ämneskategori:vet swepub-contenttype
-
Ämneskategori:art swepub-publicationtype
Anmärkningar
-
We conducted the recombination and sister chromatid exchange (SCE) assays with five chemicals (hydroxyurea (HU), resveratrol, 4-hydroxy-trans-stilbene, 3-hydroxy-trans-stilbene, and mitomycin C) in Chinese hamster cell line SPD8/V79 to confirm directly that SCE is a result of homologous recombination (HR). SPD8 has a partial duplication in exon 7 of the endogenous hprt gene and can revert to wild type by homologous recombination. All chemicals were positive in both assays except for 3-hydroxy-trans-stilbene, which was negative in both. HU, resveratrol, and 4-hydroxy-trans-stilbene were scavengers of the tyrosyl free radical of the R2 subunit of mammalian ribonucleotide reductase. Tyrosyl free radical scavengers disturb normal DNA replication, causing replication fork arrest. Mitomycin C is a DNA cross-linking agent that also causes replication fork arrest. The present study suggests that replication fork arrest, which is similar to the early phases of HR, leads to a high frequency of recombination, resulting in SCEs. The findings show that SCE may be mediated by HR.
Ämnesord och genrebeteckningar
-
Alkylating Agents/toxicity
-
Animals
-
CHO Cells
-
Cricetinae
-
Cricetulus
-
Crossing Over; Genetic/*drug effects
-
DNA Replication
-
Enzyme Inhibitors/toxicity
-
Hydroxyurea/toxicity
-
Mitomycin/toxicity
-
Protein Subunits/drug effects
-
Ribonucleotide Reductases/*antagonists & inhibitors/chemistry/drug effects
-
Sister Chromatid Exchange
-
Stilbenes/toxicity
-
Structure-Activity Relationship
-
Time Factors
Biuppslag (personer, institutioner, konferenser, titlar ...)
-
Lundin, Cecilia
(författare)
-
Johansson, Fredrik
(författare)
-
Sahlin, MargaretaStockholms universitet,Institutionen för molekylärbiologi och funktionsgenomik(Swepub:su)msahl
(författare)
-
Fukuhara, Kiyoshi
(författare)
-
Sjöberg, Britt-MarieStockholms universitet,Institutionen för molekylärbiologi och funktionsgenomik(Swepub:su)bsjob
(författare)
-
Jenssen, Dag
(författare)
-
Önfelt, Agneta
(författare)
-
Stockholms universitetInstitutionen för molekylärbiologi och funktionsgenomik
(creator_code:org_t)
Sammanhörande titlar
-
Ingår i:Mutat Res547:1-2, s. 101-70027-5107
Internetlänk
Hitta via bibliotek
Till lärosätets databas