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Vaccine based on fo...
Vaccine based on folded RBD-PreS fusion protein with potential to induce sterilizing immunity to SARS-CoV-2 variants
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Gattinger, Pia (författare)
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Kratzer, Bernhard (författare)
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Tulaeva, Inna (författare)
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Niespodziana, Katarzyna (författare)
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Ohradanova-Repic, Anna (författare)
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Gebetsberger, Laura (författare)
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Borochova, Kristina (författare)
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Garner-Spitzer, Erika (författare)
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Trapin, Doris (författare)
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- Hofer, Gerhard (författare)
- Stockholms universitet,Institutionen för material- och miljökemi (MMK)
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Keller, Walter (författare)
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Baumgartner, Isabella (författare)
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Tancevski, Ivan (författare)
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Khaitov, Musa (författare)
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Karaulov, Alexander (författare)
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Stockinger, Hannes (författare)
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Wiedermann, Ursula (författare)
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Pickl, Winfried F. (författare)
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Valenta, Rudolf (författare)
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(creator_code:org_t)
- 2022-04-15
- 2022
- Engelska.
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Ingår i: Allergy. European Journal of Allergy and Clinical Immunology. - : Wiley. - 0105-4538 .- 1398-9995. ; 77:8, s. 2431-2445
- Relaterad länk:
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https://doi.org/10.1...
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https://urn.kb.se/re...
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https://doi.org/10.1...
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Abstract
Ämnesord
Stäng
- Background: Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is responsible for the ongoing global COVID-19 pandemic. One possibility to control the pandemic is to induce sterilizing immunity through the induction and maintenance of neutralizing antibodies preventing SARS-CoV-2 from entering human cells to replicate in.Methods: We report the construction and in vitro and in vivo characterization of a SARS-CoV-2 subunit vaccine (PreS-RBD) based on a structurally folded recombinant fusion protein consisting of two SARS-CoV-2 Spike protein receptor-binding domains (RBD) fused to the N- and C-terminus of hepatitis B virus (HBV) surface antigen PreS to enable the two unrelated proteins serving as immunologic carriers for each other.Results: PreS-RBD, but not RBD alone, induced a robust and uniform RBD-specific IgG response in rabbits. Currently available genetic SARS-CoV-2 vaccines induce mainly transient IgG1 responses in vaccinated subjects whereas the PreS-RBD vaccine induced RBD-specific IgG antibodies consisting of an early IgG1 and sustained IgG4 antibody response in a SARS-CoV-2 naive subject. PreS-RBD-specific IgG antibodies were detected in serum and mucosal secretions, reacted with SARS-CoV-2 variants, including the omicron variant of concern and the HBV receptor-binding sites on PreS of currently known HBV genotypes. PreS-RBD-specific antibodies of the immunized subject more potently inhibited the interaction of RBD with its human receptor ACE2 and their virus-neutralizing titers (VNTs) were higher than median VNTs in a random sample of healthy subjects fully immunized with registered SARS-CoV-2 vaccines or in COVID-19 convalescent subjects.Conclusion: The PreS-RBD vaccine has the potential to serve as a combination vaccine for inducing sterilizing immunity against SARS-CoV-2 and HBV by stopping viral replication through the inhibition of cellular virus entry.
Ämnesord
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinska och farmaceutiska grundvetenskaper -- Immunologi inom det medicinska området (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Basic Medicine -- Immunology in the medical area (hsv//eng)
Nyckelord
- antibody response
- COVID-19
- neutralizing antibodies
- SARS-CoV-2
- sterilizing immunity
- vaccine
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
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Till lärosätets databas
- Av författaren/redakt...
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Gattinger, Pia
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Kratzer, Bernhar ...
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Tulaeva, Inna
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Niespodziana, Ka ...
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Ohradanova-Repic ...
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Gebetsberger, La ...
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visa fler...
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Borochova, Krist ...
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Garner-Spitzer, ...
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Trapin, Doris
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Hofer, Gerhard
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Keller, Walter
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Baumgartner, Isa ...
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Tancevski, Ivan
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Khaitov, Musa
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Karaulov, Alexan ...
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Stockinger, Hann ...
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Wiedermann, Ursu ...
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Pickl, Winfried ...
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Valenta, Rudolf
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visa färre...
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Allergy. Europea ...
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Stockholms universitet