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Functional design o...
Functional design of bacterial superoxide : quinone oxidoreductase
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Abou-Hamdan, Abbas (författare)
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Mahler, Roman (författare)
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Grossenbacher, Philipp (författare)
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Biner, Olivier (författare)
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- Sjöstrand, Dan (författare)
- Stockholms universitet,Institutionen för biokemi och biofysik
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Lochner, Martin (författare)
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- Högbom, Martin (författare)
- Stockholms universitet,Institutionen för biokemi och biofysik
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von Ballmoos, Christoph (författare)
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(creator_code:org_t)
- Elsevier BV, 2022
- 2022
- Engelska.
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Ingår i: Biochimica et Biophysica Acta - Bioenergetics. - : Elsevier BV. - 0005-2728 .- 1879-2650. ; 1863:7
- Relaterad länk:
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https://doi.org/10.1...
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https://urn.kb.se/re...
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https://doi.org/10.1...
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Abstract
Ämnesord
Stäng
- The superoxide anion - molecular oxygen reduced by a single electron - is produced in large amounts by enzymatic and adventitious reactions. It can perform a range of cellular functions, including bacterial warfare and iron uptake, signalling and host immune response in eukaryotes. However, it also serves as precursor for more deleterious species such as the hydroxyl anion or peroxynitrite and defense mechanisms to neutralize superoxide are important for cellular health. In addition to the soluble proteins superoxide dismutase and superoxide reductase, recently the membrane embedded diheme cytochrome b561 (CybB) from E. coli has been proposed to act as a superoxide:quinone oxidoreductase. Here, we confirm superoxide and cellular ubiquinones or menaquinones as natural substrates and show that quinone binding to the enzyme accelerates the reaction with superoxide. The reactivity of the substrates is in accordance with the here determined midpoint potentials of the two b hemes (+48 and -23 mV / NHE). Our data suggest that the enzyme can work near the diffusion limit in the forward direction and can also catalyse the reverse reaction efficiently under physiological conditions. The data is discussed in the context of described cytochrome b561 proteins and potential physiological roles of CybB.
Ämnesord
- NATURVETENSKAP -- Biologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences (hsv//eng)
Nyckelord
- Membrane protein
- Superoxide
- Ubiquinone
- Menaquinone
- Superoxide oxidase
- Cytochrome b 561
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
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