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Sökning: onr:"swepub:oai:DiVA.org:su-210681" > In Vitro Evaluation...

LIBRIS Formathandbok  (Information om MARC21)
FältnamnIndikatorerMetadata
00004050naa a2200385 4500
001oai:DiVA.org:su-210681
003SwePub
008221114s2022 | |||||||||||000 ||eng|
0247 a https://urn.kb.se/resolve?urn=urn:nbn:se:su:diva-2106812 URI
0247 a https://doi.org/10.1155/2022/12799612 DOI
040 a (SwePub)su
041 a engb eng
042 9 SwePub
072 7a ref2 swepub-contenttype
072 7a art2 swepub-publicationtype
1001 a Kalsoom, Aasia4 aut
2451 0a In Vitro Evaluation of Cytotoxic Potential of Caladium lindenii Extracts on Human Hepatocarcinoma HepG2 and Normal HEK293T Cell Lines
264 1b Hindawi Limited,c 2022
338 a print2 rdacarrier
520 a Data regarding the therapeutic potential of Caladium lindenii (C. lindenii) are insufficient. It becomes more important to explore plants as an alternative or palliative therapeutics in deadly diseases around the globe. The current study was planned to explore C. lindenii for its anticancer activity of ethanolic and hexane extracts of C. lindenii leaves against hepatic carcinoma (HepG2) and human embryonic kidney (HEK293T) cell lines. HepG2 and HEK293T cells were treated with 10, 50, 100, 200, and 400 μg/mL of ethanolic and hexane extracts of C. lindenii and were incubated for 72 h. Antiproliferative activity was measured by 3-(4,5-dimethylthiazol-2yl)-2,5-biphenyl tetrazolium bromide (MTT) assay, and percentage viability were calculated through crystal violet staining and cellular morphology by Floid Cell Imaging Station. The study showed ethanolic extract exhibiting a significantly higher antiproliferative effect on HepG2 (IC50=31 μg/mL) in a concentration-dependent manner, while HEK293T (IC50=241 μg/mL) cells showed no toxicity. Hexane extract exhibited lower cytotoxicity (IC50=150 μg/mL) on HepG2 cells with no effect on HEK293T (IC50=550 μg/mL). On the other hand, the percentage viability of HepG2 cells was recorded as 78%, 67%, 50%, 37%, and 28% by ethanolic extracts, and 88%, 80%, 69%, 59%, and 50% by hexane extracts at tested concentrations of both extracts. Toxicity assay showed significantly safer ranges of percentage viabilities in normal cells (HEK293T), i.e., 95%, 90%, 88%, 76%, and 61% with ethanolic extract and 97%, 95%, 88%, 75%, and 62% with hexane extract. The assay validity revealed 100% viability in the control negative (dimethyl sulfoxide treated) and less than 45% in the control positive (cisplatin) on both HepG2 and HEK293T cells. Morphological studies showed alterations in HepG2 cells upon exposure to >50 μg/mL of ethanolic extracts and ≥400 μg/mL of hexane extracts. HEK293T on the other hand did not change its morphology against any of the extracts compared to the aggressive changes on the HepG2 cell line by both extracts and positive control (cisplatin). In conclusion, extracts of C. lindenii are proved to have significant potential for cytotoxicity-induced apoptosis in human cancer HepG2 cells and are less toxic to normal HEK293T cells. Hence C. lindenii extracts are proposed to be used against hepatocellular carcinoma (HCC) after further validations.
650 7a NATURVETENSKAPx Biologi0 (SwePub)1062 hsv//swe
650 7a NATURAL SCIENCESx Biological Sciences0 (SwePub)1062 hsv//eng
7001 a Altaf, Awais4 aut
7001 a Ashraf, Muhammad4 aut
7001 a Ali, Muhammad Muddassir4 aut
7001 a Aftab, Sairau Stockholms universitet,Institutionen för biokemi och biofysik4 aut0 (Swepub:su)saaf2354
7001 a Sattar, Huma4 aut
7001 a Sajjad, Muhammad4 aut
7001 a Aqib, Amjad Islam4 aut
7001 a Maqbool, Tahir4 aut
7102 a Stockholms universitetb Institutionen för biokemi och biofysik4 org
7730 t BioMed Research Internationald : Hindawi Limitedg 2022q 2022x 2314-6133x 2314-6141
8564 u https://doi.org/10.1155/2022/1279961y Fulltext
8564 8u https://urn.kb.se/resolve?urn=urn:nbn:se:su:diva-210681
8564 8u https://doi.org/10.1155/2022/1279961

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