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Sökning: onr:"swepub:oai:DiVA.org:su-226920" > Botulinum neurotoxi...

LIBRIS Formathandbok  (Information om MARC21)
FältnamnIndikatorerMetadata
00003190naa a2200433 4500
001oai:DiVA.org:su-226920
003SwePub
008240229s2024 | |||||||||||000 ||eng|
0247 a https://urn.kb.se/resolve?urn=urn:nbn:se:su:diva-2269202 URI
0247 a https://doi.org/10.1128/mbio.03106-232 DOI
040 a (SwePub)su
041 a engb eng
042 9 SwePub
072 7a ref2 swepub-contenttype
072 7a art2 swepub-publicationtype
1001 a Gregg, Brieana M.4 aut
2451 0a Botulinum neurotoxin X lacks potency in mice and in human neurons
264 1c 2024
338 a print2 rdacarrier
520 a Botulinum neurotoxins (BoNTs) are a class of toxins produced by Clostridium botulinum (C. botulinum) and other species of Clostridia. BoNT/X is a putative novel botulinum neurotoxin identified through genome sequencing and capable of SNARE cleavage, but its neurotoxic potential in humans and vertebrates remained unclear. The C. botulinum strain producing BoNT/X, Strain 111, encodes both a plasmid-borne bont/b2 as well as the chromosomal putative bont/x. This study utilized C. botulinum Strain 111 from Japan as well as recombinantly produced full-length BoNT/X to more fully analyze this putative pathogenic toxin. We confirmed production of full-length, catalytically active native BoNT/X by C. botulinum Strain 111, produced as a disulfide-bonded dichain polypeptide similar to other BoNTs. Both the purified native and the recombinant BoNT/X had high enzymatic activity in vitro but displayed very low potency in human-induced pluripotent stem cell-derived neuronal cells and in mice. Intraperitoneal injection of up to 50 µg of native BoNT/X in mice did not result in botulism; however, mild local paralysis was observed after injection of 2 μg into the gastrocnemius muscle. We further demonstrate that the lack of toxicity by BoNT/X is due to inefficient neuronal cell association and entry, which can be rescued by replacing the receptor binding domain of BoNT/X with that of BoNT/A. These data demonstrate that BoNT/X is not a potent vertebrate neurotoxin like the classical seven serotypes of BoNTs.
650 7a NATURVETENSKAPx Biologix Biokemi och molekylärbiologi0 (SwePub)106022 hsv//swe
650 7a NATURAL SCIENCESx Biological Sciencesx Biochemistry and Molecular Biology0 (SwePub)106022 hsv//eng
653 a botulinum neurotoxin
653 a BoNT
653 a Clostridium botulinum
653 a BoNT/X
7001 a Matsumura, Takuhiro4 aut
7001 a Wentz, Travis G.4 aut
7001 a Tepp, William H.4 aut
7001 a Bradshaw, Marite4 aut
7001 a Stenmark, Pål,d 1976-u Stockholms universitet,Institutionen för biokemi och biofysik4 aut0 (Swepub:su)stenm
7001 a Johnson, Eric A.4 aut
7001 a Fujinaga, Yukako4 aut
7001 a Pellett, Sabine4 aut
7102 a Stockholms universitetb Institutionen för biokemi och biofysik4 org
7730 t mBiog 15:3q 15:3x 2161-2129x 2150-7511
8564 u https://doi.org/10.1128/mbio.03106-23y Fulltext
8564 8u https://urn.kb.se/resolve?urn=urn:nbn:se:su:diva-226920
8564 8u https://doi.org/10.1128/mbio.03106-23

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