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Elucidation of the ...
Elucidation of the O-antigen structure of Escherichia coli O93 and characterization of its biosynthetic genes
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- Furevi, Axel, 1992- (författare)
- Stockholms universitet,Institutionen för organisk kemi
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- Ståhle, Jonas, 1985- (författare)
- Stockholms universitet,Institutionen för organisk kemi
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- Muheim, Claudio, 1987- (författare)
- Stockholms universitet,Institutionen för biokemi och biofysik
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- Gkotzis, Spyridon (författare)
- Stockholms universitet,Institutionen för molekylär biovetenskap, Wenner-Grens institut
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- Daley, Daniel (författare)
- Stockholms universitet,Institutionen för biokemi och biofysik
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- Udekwu, Klas (författare)
- Stockholms universitet,Institutionen för molekylär biovetenskap, Wenner-Grens institut,Swedish University of Agriculture, Sweden
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- Widmalm, Göran, 1958- (författare)
- Stockholms universitet,Institutionen för organisk kemi
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(creator_code:org_t)
- 2022-10-14
- 2023
- Engelska.
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Ingår i: Glycobiology. - : Oxford University Press (OUP). - 0959-6658 .- 1460-2423. ; 33:4, s. 289-300
- Relaterad länk:
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https://doi.org/10.1...
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https://urn.kb.se/re...
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https://doi.org/10.1...
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Abstract
Ämnesord
Stäng
- The structure of the O-antigen from the international reference strain Escherichia coli O93:-:H16 has been determined. A nonrandom modal chain-length distribution was observed for the lipopolysaccharide, a pattern which is typical when long O-specific polysaccharides are expressed. By a combination of (i) bioinformatics information on the gene cluster related to O-antigen synthesis including putative function on glycosyl transferases, (ii) the magnitude of NMR coupling constants of anomeric protons, and (iii) unassigned 2D H-1, C-13-HSQC, and H-1,H-1-TOCSY NMR spectra it was possible to efficiently elucidate the structure of the carbohydrate polymer in an automated fashion using the computer program CASPER. The polysaccharide also carries O-acetyl groups and their locations were determined by 2D NMR experiments showing that similar to 1/2 of the population was 2,6-di-O-acetylated, similar to 1/4 was 2-O-acetylated, whereas similar to 1/4 did not carry O-acetyl group(s) in the 3-O-substituted mannosyl residue of the repeating unit. The structure of the tetrasaccharide repeating unit of the O-antigen is given by: -> 2)-beta-D-Manp-(1 -> 3)-beta-D-Manp2Ac6Ac-(1 -> 4)-beta-D-GlcpA-(1 -> 3)-alpha-D-GlcpNAc-(1 ->, which should also be the biological repeating unit and it shares structural elements with capsular polysaccharides from E. coli K84 and K50. The structure of the acidic O-specific polysaccharide from Cellulophaga baltica strain NN015840(T) differs to that of the O-antigen from E. coli O93 by lacking the O-acetyl group at O6 of the O-acetylated mannosyl residue.
Ämnesord
- NATURVETENSKAP -- Biologi -- Biokemi och molekylärbiologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Biochemistry and Molecular Biology (hsv//eng)
Nyckelord
- bioinformatics
- CarbBuilder
- CASPER
- lipopolysaccharide
- NMR spectroscopy
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
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Furevi, Axel, 19 ...
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Ståhle, Jonas, 1 ...
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Muheim, Claudio, ...
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Gkotzis, Spyrido ...
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Daley, Daniel
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Udekwu, Klas
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Widmalm, Göran, ...
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- Om ämnet
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- NATURVETENSKAP
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NATURVETENSKAP
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och Biologi
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och Biokemi och mole ...
- Artiklar i publikationen
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Glycobiology
- Av lärosätet
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Stockholms universitet