Sökning: onr:"swepub:oai:DiVA.org:su-25188" >
Impaired Toll-like ...
-
Amoudruz, PetraStockholms universitet,Wenner-Grens institut
(författare)
Impaired Toll-like receptor 2 signaling in monocytes from 5-year-old allergic children
- Artikel/kapitelEngelska2009
Förlag, utgivningsår, omfång ...
-
2009-02-02
-
Oxford University Press (OUP),2009
-
printrdacarrier
Nummerbeteckningar
-
LIBRIS-ID:oai:DiVA.org:su-25188
-
https://urn.kb.se/resolve?urn=urn:nbn:se:su:diva-25188URI
-
https://doi.org/10.1111/j.1365-2249.2008.03792.xDOI
-
http://kipublications.ki.se/Default.aspx?queryparsed=id:118212845URI
Kompletterande språkuppgifter
-
Språk:engelska
-
Sammanfattning på:engelska
Ingår i deldatabas
Klassifikation
-
Ämneskategori:ref swepub-contenttype
-
Ämneskategori:art swepub-publicationtype
Anmärkningar
-
The relative composition of the two major monocytic subsets CD14+CD16− and CD14+CD16+ is altered in some allergic diseases. These two subsets display different patterns of Toll-like receptor levels, which could have implications for activation of innate immunity leading to reduced immunoglobulin E-specific adaptive immune responses. This study aimed to investigate if allergic status at the age of 5 years is linked to differences in monocytic subset composition and their Toll-like receptor levels, and further, to determine if Toll-like receptor regulation and cytokine production upon microbial stimuli is influenced by the allergic phenotype. Peripheral blood mononuclear cells from 5-year-old allergic and non-allergic children were stimulated in vitro with lipopolysaccharide and peptidoglycan. Cells were analysed with flow cytometry for expression of CD14, Toll-like receptors 2 and 4 and p38-mitogen-activated protein kinase (MAPK). The release of cytokines and chemokines [tumour necrosis factor, interleukin (IL)-1β, IL-6, IL-8, IL-10, IL-12p70] into culture supernatants was measured with cytometric bead array. For unstimulated cells there were no differences in frequency of the monocytic subsets or their Toll-like receptor levels between allergic and non-allergic children. However, monocytes from allergic children had a significantly lower up-regulation of Toll-like receptor 2 upon peptidoglycan stimulation. Further, monocytes from allergic children had a higher spontaneous production of IL-6, but there were no differences between the two groups regarding p38-MAPK activity or cytokine and chemokine production upon stimulation. The allergic subjects in this study have a monocytic population that seems to display a hyporesponsive state as implicated by impaired regulation of Toll-like receptor 2 upon peptidoglycan stimulation.
Ämnesord och genrebeteckningar
Biuppslag (personer, institutioner, konferenser, titlar ...)
-
Holmlund, UlrikaStockholms universitet,Wenner-Grens institut(Swepub:su)ulho0917
(författare)
-
Saghafian-Hedengren, ShanieStockholms universitet,Wenner-Grens institut(Swepub:su)shsa1905
(författare)
-
Nilsson, CarolineKarolinska Institutet
(författare)
-
Sverremark-Ekström, EvaStockholms universitet,Wenner-Grens institut(Swepub:su)esver
(författare)
-
Stockholms universitetWenner-Grens institut
(creator_code:org_t)
Sammanhörande titlar
-
Ingår i:Clinical and Experimental Immunology: Oxford University Press (OUP)155:3, s. 387-3940009-91041365-2249
Internetlänk
Hitta via bibliotek
Till lärosätets databas