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Targeting cytokine ...
Targeting cytokine expression in glial cells by cellular delivery of an NFκB decoy
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- Fisher, Linda (författare)
- Stockholms universitet,Institutionen för neurokemi
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- Samuelsson, Malin (författare)
- Stockholms universitet,Institutionen för neurokemi
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- Jiang, Yang (författare)
- Stockholms universitet,Institutionen för neurokemi
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- Ramberg, Veronica (författare)
- Stockholms universitet,Institutionen för neurokemi
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- Figueroa, Ricardo (författare)
- Södertörns högskola,Institutionen för livsvetenskaper
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- Hallberg, Einar (författare)
- Södertörns högskola,Institutionen för livsvetenskaper
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- Langel, Ülo (författare)
- Stockholms universitet,Institutionen för neurokemi
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- Iverfeldt, Kerstin (författare)
- Stockholms universitet,Institutionen för neurokemi
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(creator_code:org_t)
- 2007
- 2007
- Engelska.
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Ingår i: Journal of Molecular Neuroscience. - 0895-8696 .- 1559-1166. ; 31:3, s. 209-219
- Relaterad länk:
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https://urn.kb.se/re...
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https://doi.org/10.1...
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https://urn.kb.se/re...
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Abstract
Ämnesord
Stäng
- Inhibition of nuclear factor (NF)-κB has emerged as an important strategy for design of anti-inflammatory therapies. In neurodegenerative disorders like Alzheimer’s disease, inflammatory reactions mediated by glial cells are believed to promote disease progression. Here, we report that uptake of a double-stranded oligonucleotide NF-κB decoy in rat primary glial cells is clearly facilitated by noncovalent binding to a cell-penetrating peptide, transportan 10, via a complementary peptide nucleic acid (PNA) sequence. Fluorescently labeled oligonucleotide decoy was detected in the cells within 1 h only when cells were incubated with the decoy in the presence of cell-penetrating peptide. Cellular delivery of the decoy also inhibited effects induced by a neurotoxic fragment of the Alzheimer β amyloid peptide in the presence of the inflammatory cytokine interleukin (IL) 1β. Pretreatment of the cells with the complex formed by the decoy and the cell-penetrating peptide-PNA resulted in 80% and 50% inhibition of the NF-κB binding activity and IL-6 mRNA expression, respectively.
Ämnesord
- NATURVETENSKAP -- Biologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences (hsv//eng)
- NATURVETENSKAP -- Biologi -- Biokemi och molekylärbiologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Biochemistry and Molecular Biology (hsv//eng)
- MEDICIN OCH HÄLSOVETENSKAP -- Klinisk medicin -- Neurologi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Clinical Medicine -- Neurology (hsv//eng)
Nyckelord
- β-amyloid
- astrocytes
- cell-penetrating peptide
- inflammation
- nuclear factor-κB
- peptide nucleic acid
- Biochemistry
- biokemi
- Neurochemistry and Neurotoxicology
- neurokemi och neurotoxikologi
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
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