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Down-regulation of ...
Down-regulation of amyloid precursor protein by peptide nucleic acid oligomer in cultured rat primary neurons and astrocytes
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- Adlerz, Linda (author)
- Stockholms universitet,Institutionen för neurokemi och neurotoxikologi
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- Soomets, Ursel (author)
- Stockholms universitet,Institutionen för neurokemi och neurotoxikologi,University of Tartu, Estonia
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- Holmlund, Linda (author)
- Stockholms universitet,Institutionen för neurokemi och neurotoxikologi
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Virland, Saade (author)
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- Langel, Ülo (author)
- Stockholms universitet,Institutionen för neurokemi och neurotoxikologi
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- Iverfeldt, Kerstin (author)
- Stockholms universitet,Institutionen för neurokemi och neurotoxikologi
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(creator_code:org_t)
- 2003
- 2003
- English.
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In: Neuroscience Letters. - 0304-3940 .- 1872-7972. ; 336:1, s. 55-59
- Related links:
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https://urn.kb.se/re...
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https://doi.org/10.1...
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Abstract
Subject headings
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- The amyloid precursor protein (APP) and its proteolytic cleavage products, the amyloid P peptides, have been implicated as a cause of Alzheimer's disease. Peptide nucleic acids (PNA), the DNA mimics, have been shown to block the expression of specific proteins at both transcriptional and translational levels. Generally, the cellular uptake of PNA is low. However, recent studies have indicated that the effect of unmodified antisense PNA uptake is more pronounced in nervous tissue. In this study we have shown that biotinylated PNA directed to the initiator codon region of the APP mRNA (-4 - +11) was taken up into the cytoplasm of primary rat cerebellar granule cells and cortical astrocytes, using fluorescence and confocal microscopy studies. Uptake of PNA was faster in neurons than in astrocytes. Western blotting analysis showed that APP was strongly down-regulated in both neurons and astrocytes. Thus, unmodified PNA can be used for studies on the function of APP in neurons and astrocytes.
Subject headings
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinska och farmaceutiska grundvetenskaper -- Neurovetenskaper (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Basic Medicine -- Neurosciences (hsv//eng)
- MEDICIN OCH HÄLSOVETENSKAP -- Klinisk medicin -- Neurologi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Clinical Medicine -- Neurology (hsv//eng)
Keyword
- Alzheimer's disease
- amyloid precursor protein
- antisense
- cerebellar granule cells
- peptide nucleic acid
Publication and Content Type
- ref (subject category)
- art (subject category)
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