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Increased fatigue r...
Increased fatigue resistance linked to Ca(2+)-stimulated mitochondrial biogenesis in muscle fibres of cold-acclimated mice
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- Bruton, Joseph D. (författare)
- Karolinska Institutet
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- Aydin, Jan (författare)
- Karolinska Institutet
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Yamada, Takashi (författare)
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- Shabalina, Irina G. (författare)
- Stockholms universitet,Avdelningen för fysiologi
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- Ivarsson, Niklas (författare)
- Karolinska Institutet
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- Zhang, Shi-Jin (författare)
- Karolinska Institutet
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Wada, Masanobu (författare)
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Tavi, Pasi (författare)
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- Nedergaard, Jan (författare)
- Stockholms universitet,Avdelningen för fysiologi
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- Katz, Abram (författare)
- Karolinska Institutet
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- Westerblad, Håkan (författare)
- Karolinska Institutet
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(creator_code:org_t)
- 2010-10-29
- 2010
- Engelska.
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Ingår i: Journal of Physiology. - : Wiley. - 0022-3751 .- 1469-7793. ; 588:21, s. 4275-4288
- Relaterad länk:
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https://physoc.onlin...
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https://urn.kb.se/re...
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https://doi.org/10.1...
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Abstract
Ämnesord
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- Mammals exposed to a cold environment initially generate heat by repetitive muscle activity (shivering). Shivering is successively replaced by the recruitment of uncoupling protein-1 (UCP1)-dependent heat production in brown adipose tissue. Interestingly, adaptations observed in skeletal muscles of cold-exposed animals are similar to those observed with endurance training. We hypothesized that increased myoplasmic free [Ca2+] ([Ca2+]i) is important for these adaptations. To test this hypothesis, experiments were performed on flexor digitorum brevis (FDB) muscles, which do not participate in the shivering response, of adult wild-type (WT) and UCP1-ablated (UCP1-KO) mice kept either at room temperature (24 ºC) or cold-acclimated (4 ºC) for 4-5 weeks. [Ca2+]i (measured with indo-1) and force were measured under control conditions and during fatigue induced by repeated tetanic stimulation in intact single fibres. The results show no differences between fibres from WT and UCP1-KO mice. However, muscle fibres from cold-acclimated mice showed significant increases in basal [Ca2+]i (~50%), tetanic [Ca2+]i (~40%), and sarcoplasmic reticulum (SR) Ca2+ leak (~four-fold) as compared to fibres from room-temperature mice. Muscles of cold-acclimated mice showed increased expression of peroxisome proliferator-activated receptor-γ coactivator-1α (PGC-1α) and increased citrate synthase activity (reflecting increased mitochondrial content). Fibres of cold-acclimated mice were more fatigue resistant with higher tetanic [Ca2+]i and less force loss during fatiguing stimulation. In conclusion, cold exposure induces changes in FDB muscles similar to those observed with endurance training and we propose that increased [Ca2+]i is a key factor underlying these adaptations.
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- art (ämneskategori)
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Till lärosätets databas
- Av författaren/redakt...
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Bruton, Joseph D ...
-
Aydin, Jan
-
Yamada, Takashi
-
Shabalina, Irina ...
-
Ivarsson, Niklas
-
Zhang, Shi-Jin
-
visa fler...
-
Wada, Masanobu
-
Tavi, Pasi
-
Nedergaard, Jan
-
Katz, Abram
-
Westerblad, Håka ...
-
visa färre...
- Artiklar i publikationen
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Journal of Physi ...
- Av lärosätet
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Stockholms universitet
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Karolinska Institutet