Testing membrane interactions of CPPs

• The chapter deals with some biophysical methods used for investigating CPP-induced changes in membrane properties by spectroscopy methods such as fluorescence or NMR and methods used for probing CPP-induced leakage in membranes. Some useful model systems for biomembranes are described. These include large unilamellar phospholipid vesicles (LUVs) of well-defined size (diameter typically 100 nm). A protocol for the preparation of such vesicles is included. The leakage studies make use of LUVs with entrapped dye molecules. The NMR studies make use of mixed micelles (bicelles) as a membrane mimetic system, which can be oriented in the magnetic field of the spectrometer.

Nyckelord

Fluorescence

NMR

Large unilamellar vesicles

Bicelles

Membrane leakage

Membrane fluidity

Membrane dynamics

NATURAL SCIENCES

NATURVETENSKAP

Biophysics

biofysik

Biochemistry

biokemi

Publikations- och innehållstyp

ref (ämneskategori)

art (ämneskategori)