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Sökning: onr:"swepub:oai:DiVA.org:su-57718" > GVHD after chemothe...

  • Sadeghi, B.Karolinska Institutet (författare)

GVHD after chemotherapy conditioning in allogeneic transplanted mice

  • Artikel/kapitelEngelska2008

Förlag, utgivningsår, omfång ...

  • 2008-09-29
  • Springer Science and Business Media LLC,2008
  • printrdacarrier

Nummerbeteckningar

  • LIBRIS-ID:oai:DiVA.org:su-57718
  • https://urn.kb.se/resolve?urn=urn:nbn:se:su:diva-57718URI
  • https://doi.org/10.1038/bmt.2008.261DOI
  • http://kipublications.ki.se/Default.aspx?queryparsed=id:117985643URI

Kompletterande språkuppgifter

  • Språk:engelska
  • Sammanfattning på:engelska

Ingår i deldatabas

Klassifikation

  • Ämneskategori:ref swepub-contenttype
  • Ämneskategori:art swepub-publicationtype

Anmärkningar

  • authorCount :7
  • GVHD is a major complication in allogeneic SCT. Available GVHD models are mainly based on radio-therapy-conditioning and/or immune deficient mice. GVHD models based on chemotherapy-based regimens remain poorly studied, despite 50% of all transplantations being chemotherapy based. Our aim was to develop a GVHD model using chemotherapy as conditioning. Female BALB/c (H-2Kd) were conditioned with BU-CY and transplanted with 2 x 10(7) BM and 3 x 10(7) spleen cells from either C57BL/6 (H-2Kb) mice ( allogeneic setting) or from male BALB/c to serve as a control group for regimen-related toxicity and engraftment. GVHD manifestations and histopathological changes were evaluated. Chimerism and donor T cells presence in skin, intestine and liver were studied using FACS-, FISH analysis and immunohistochemistry. Allogeneic transplanted mice developed lethal GVHD starting from day+7 with both histological and clinical signs. Donor T cells accumulated in recipient skin and intestine with GVHD progression. BM-failure, apoptosis and T-lymphocyte infiltration into target organs were significantly higher in allogeneic when compared with the syngeneic group. No toxicity or GVHD signs were observed in the syngeneic setting. We report a mouse model of GVHD using BU-CY conditioning that represents the most common myeloablative-conditioning regimen in clinical SCT. This model can be utilized to study the role of conditioning on mechanisms underlying GVHD.

Ämnesord och genrebeteckningar

  • BU
  • CY
  • chemotherapy conditioning
  • GVHD
  • mouse model
  • hematopoietic stem cell transplantation
  • versus-host-disease
  • bone-marrow-transplantation
  • total-body irradiation
  • minor histocompatibility antigens
  • stem-cell transplantation
  • acute myeloid-leukemia
  • t-cells
  • in-vivo
  • randomized-trial
  • lethal graft
  • NATURAL SCIENCES
  • NATURVETENSKAP

Biuppslag (personer, institutioner, konferenser, titlar ...)

  • Aghdami, N. (författare)
  • Hassan, Z.Karolinska Institutet (författare)
  • Forouzanfar, M. (författare)
  • Rozell, B.Karolinska Institutet (författare)
  • Abedi-Valugerdi, ManuchehrKarolinska Institutet,Stockholms universitet,Institutionen för biokemi och biofysik(Swepub:su)mabed (författare)
  • Hassan, M.Karolinska Institutet (författare)
  • Karolinska InstitutetInstitutionen för biokemi och biofysik (creator_code:org_t)

Sammanhörande titlar

  • Ingår i:Bone Marrow Transplantation: Springer Science and Business Media LLC42:12, s. 807-8180268-33691476-5365

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