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Mutations in 16S rR...
Mutations in 16S rRNA that suppress cold-sensitive initiation factor 1 affect ribosomal subunit association
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- Belotserkovsky, Jaroslav M., 1980- (författare)
- Stockholms universitet,Institutionen för genetik, mikrobiologi och toxikologi
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Dabbs, Eric R. (författare)
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- Isaksson, Leif A. (författare)
- Stockholms universitet,Institutionen för genetik, mikrobiologi och toxikologi
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(creator_code:org_t)
- 2011-09-05
- 2011
- Engelska.
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Ingår i: The FEBS Journal. - : Wiley. - 1742-464X .- 1742-4658. ; 278:18, s. 3508-3517
- Relaterad länk:
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https://febs.onlinel...
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https://urn.kb.se/re...
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https://doi.org/10.1...
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Abstract
Ämnesord
Stäng
- A mutation in the infA gene encoding initiation factor 1 (IF1) gives rise to a cold-sensitive phenotype. An Escherichia coli strain with this mutation was used as a tool to select for second-site suppressors that compensate for the cold sensitivity and map specifically to rRNA. Several suppressor mutants with altered 16S rRNA that partially restore growth of an IF1 mutant strain in the cold were isolated and characterized. Suppressor mutations were found in helix (h) 18, h32, h34 and h41 in 16S rRNA. These mutations are not clustered to any particular region in 16S rRNA and none overlap previously reported sites of interaction with IF1. While the isolated suppressors are structurally diverse, they are functionally related because all affect ribosomal subunit association in vivo. Furthermore, in vitro subunit-association experiments indicate that most of the suppressor mutations directly influence ribosomal subunit association even though none of these are confined to any of the known intersubunit bridges. These results are consistent with the model that IF1 is an rRNA chaperone that induces large-scale conformational changes in the small ribosomal subunit, and as a consequence modulates initiation of translation by affecting subunit association.
Ämnesord
- NATURVETENSKAP -- Biologi -- Genetik (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Genetics (hsv//eng)
Nyckelord
- 16S rRNA mutant
- Escherichia coli
- IF1
- rRNA maturation
- translation
- molekylärgenetik
- Molecular Genetics
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
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