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Solid formulation o...
Solid formulation of cell-penetrating peptide nanoparticles with siRNA and their stability in simulated gastric conditions
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- Ezzat, Kariem (författare)
- Stockholms universitet,Institutionen för neurokemi
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Zaghloul, Eman M. (författare)
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EL Andaloussi, Samir (författare)
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Lehto, Taavi (författare)
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Hilal, Ramy (författare)
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- Magdy, Tarek (författare)
- Stockholms universitet,Institutionen för neurokemi
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Smith, Edvard C. I. (författare)
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- Langel, Ülo (författare)
- Stockholms universitet,Institutionen för neurokemi
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(creator_code:org_t)
- Elsevier BV, 2012
- 2012
- Engelska.
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Ingår i: Journal of Controlled Release. - : Elsevier BV. - 0168-3659 .- 1873-4995. ; 162:1, s. 1-8
- Relaterad länk:
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https://doi.org/10.1...
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https://doi.org/10.1...
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https://urn.kb.se/re...
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https://doi.org/10.1...
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Abstract
Ämnesord
Stäng
- Cell-penetrating peptides (CPPs) are short cationic peptides that have been extensively studied as drug delivery vehicles for proteins, nucleic acids and nanoparticles. However, the formulation of CPP-based therapeutics into different pharmaceutical formulations and their stability in relevant biological environments have not been given the same attention. Here, we show that a newly developed CPP, PepFect 14 (PF14), forms non-covalent nanocomplexes with short interfering RNA (siRNA), which are able to elicit efficient RNA-interference (RNAi) response in different cell-lines. RNAi effect was obtained at low siRNA doses with a unique kinetic profile. Furthermore, we utilized the solid dispersion technique to formulate PF14/siRNA nanocomplexes into solid formulations that were as active as the freshly prepared nanocomplexes in solution. Importantly, the freshly prepared nanocomplexes and solid formulations were stable after incubation with simulated gastric fluid having a pH of 1.2 and containing proteolytic enzymes. These results demonstrate the activity of PF14 in delivering and protecting siRNA in different pharmaceutical forms and biological environments.
Ämnesord
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinsk bioteknologi -- Medicinsk bioteknologi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Medical Biotechnology -- Medical Biotechnology (hsv//eng)
Nyckelord
- Cell penetrating peptide
- siRNA
- Solid formulation
- Acid stability
- Gastric fluid
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
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