Intracellular deoxyribonucleotide pool imbalance and DNA damage in cells treated with hydroxyurea, an inhibitor of ribonucleotide reductase

Golkar, Siv Österman (författare): Stockholms universitet,Institutionen för molekylär biovetenskap, Wenner-Grens institut

Czene, Stefan (författare): Stockholms universitet,Institutionen för molekylär biovetenskap, Wenner-Grens institut,AstraZeneca R&D, Safety Assessment, Dept Genet Toxicol, AstraZeneca, Södertälje, Sweden

Gokarakonda, Amulya (författare): Stockholms universitet,Institutionen för molekylär biovetenskap, Wenner-Grens institut

Haghdoost, Siamak (författare): Stockholms universitet,Institutionen för molekylär biovetenskap, Wenner-Grens institut

(creator_code:org_t)

2013-09-27
2013
Engelska.
Ingår i: Mutagenesis. - : Oxford University Press (OUP). - 0267-8357 .- 1464-3804. ; 28:6, s. 653-660

Relaterad länk:: https://academic.oup...; visa fler...; https://urn.kb.se/re...; https://doi.org/10.1...; visa färre...

Abstract Ämnesord

Stäng

Imbalance in the nucleotide pool of mammalian cells has been shown to result in genotoxic damage. The goal of this study was to devise a sensitive, reproducible and simple method for detection of nucleotide pool changes in mammalian cells that could be used for problem-solving activities in drug development, e.g. mechanistic explanation of a positive response in a mammalian in vitro genotoxicity test. The method evaluated in this study is based on ethanol extraction of the total nucleotide pool, heat treatment and filtration, treatment with calf intestine alkaline phosphatase to convert nucleotides to nucleosides and analysis of the nucleosides by high-performance liquid chromatography with ultraviolet detection. The method was applied to measure the intracellular levels of deoxyribonucleotides in mouse lymphoma (ML) L5178Y cells treated with various concentrations of a model compound, hydroxyurea (HU), a ribonucleotide reductase inhibitor. DNA strand breakage and micronuclei formation were assessed in the same experiments. Imbalance of nucleotide pool (i.e. changes in the relative ratios between individual nucleotide pools) in HU-treated ML cells has been observed already at a concentration of 0.01 mmol/l, whereas genotoxic effects became apparent only at higher concentrations of HU (i.e. 0.25 mmol/l and higher) as indicated by formation of DNA strand breaks and micronuclei.

Av författaren/redakt...: Golkar, Siv Öste ...; Czene, Stefan; Gokarakonda, Amu ...; Haghdoost, Siama ...

Om ämnet

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