onr:"swepub:oai:DiVA.org:su-97032"
Sökning: onr:"swepub:oai:DiVA.org:su-97032" > Intracellular deoxy...
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| Fältnamn | Indikatorer | Metadata |
|---|---|---|
| 000 | 03696naa a2200373 4500 | |
| 001 | oai:DiVA.org:su-97032 | |
| 003 | SwePub | |
| 008 | 131202s2013 | |||||||||||000 ||eng| | |
| 024 | 7 | a https://urn.kb.se/resolve?urn=urn:nbn:se:su:diva-970322 URI |
| 024 | 7 | a https://doi.org/10.1093/mutage/get0442 DOI |
| 040 | a (SwePub)su | |
| 041 | a engb eng | |
| 042 | 9 SwePub | |
| 072 | 7 | a ref2 swepub-contenttype |
| 072 | 7 | a art2 swepub-publicationtype |
| 100 | 1 | a Golkar, Siv Östermanu Stockholms universitet,Institutionen för molekylär biovetenskap, Wenner-Grens institut4 aut |
| 245 | 1 0 | a Intracellular deoxyribonucleotide pool imbalance and DNA damage in cells treated with hydroxyurea, an inhibitor of ribonucleotide reductase |
| 264 | c 2013-09-27 | |
| 264 | 1 | b Oxford University Press (OUP),c 2013 |
| 338 | a print2 rdacarrier | |
| 500 | a AuthorCount:4;Funding agency:AstraZeneca 2094202, 2094204 | |
| 520 | a Imbalance in the nucleotide pool of mammalian cells has been shown to result in genotoxic damage. The goal of this study was to devise a sensitive, reproducible and simple method for detection of nucleotide pool changes in mammalian cells that could be used for problem-solving activities in drug development, e.g. mechanistic explanation of a positive response in a mammalian in vitro genotoxicity test. The method evaluated in this study is based on ethanol extraction of the total nucleotide pool, heat treatment and filtration, treatment with calf intestine alkaline phosphatase to convert nucleotides to nucleosides and analysis of the nucleosides by high-performance liquid chromatography with ultraviolet detection. The method was applied to measure the intracellular levels of deoxyribonucleotides in mouse lymphoma (ML) L5178Y cells treated with various concentrations of a model compound, hydroxyurea (HU), a ribonucleotide reductase inhibitor. DNA strand breakage and micronuclei formation were assessed in the same experiments. Imbalance of nucleotide pool (i.e. changes in the relative ratios between individual nucleotide pools) in HU-treated ML cells has been observed already at a concentration of 0.01 mmol/l, whereas genotoxic effects became apparent only at higher concentrations of HU (i.e. 0.25 mmol/l and higher) as indicated by formation of DNA strand breaks and micronuclei. | |
| 650 | 7 | a MEDICIN OCH HÄLSOVETENSKAPx Medicinska och farmaceutiska grundvetenskaperx Farmakologi och toxikologi0 (SwePub)301022 hsv//swe |
| 650 | 7 | a MEDICAL AND HEALTH SCIENCESx Basic Medicinex Pharmacology and Toxicology0 (SwePub)301022 hsv//eng |
| 650 | 7 | a NATURVETENSKAPx Biologix Genetik0 (SwePub)106092 hsv//swe |
| 650 | 7 | a NATURAL SCIENCESx Biological Sciencesx Genetics0 (SwePub)106092 hsv//eng |
| 700 | 1 | a Czene, Stefanu Stockholms universitet,Institutionen för molekylär biovetenskap, Wenner-Grens institut,AstraZeneca R&D, Safety Assessment, Dept Genet Toxicol, AstraZeneca, Södertälje, Sweden4 aut0 (Swepub:su)czene |
| 700 | 1 | a Gokarakonda, Amulyau Stockholms universitet,Institutionen för molekylär biovetenskap, Wenner-Grens institut4 aut |
| 700 | 1 | a Haghdoost, Siamaku Stockholms universitet,Institutionen för molekylär biovetenskap, Wenner-Grens institut4 aut0 (Swepub:su)shagh |
| 710 | 2 | a Stockholms universitetb Institutionen för molekylär biovetenskap, Wenner-Grens institut4 org |
| 773 | 0 | t Mutagenesisd : Oxford University Press (OUP)g 28:6, s. 653-660q 28:6<653-660x 0267-8357x 1464-3804 |
| 856 | 4 | u https://academic.oup.com/mutage/article-pdf/28/6/653/4082633/get044.pdf |
| 856 | 4 8 | u https://urn.kb.se/resolve?urn=urn:nbn:se:su:diva-97032 |
| 856 | 4 8 | u https://doi.org/10.1093/mutage/get044 |
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