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Crowded, cell-like ...
Crowded, cell-like environment induces shape changes in aspherical protein
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Homouz, Dirar (författare)
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Perham, Michael (författare)
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Samiotakis, Antonios (författare)
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Cheung, Margaret S (författare)
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- Wittung-Stafshede, Pernilla (författare)
- Umeå universitet,Kemiska institutionen
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(creator_code:org_t)
- 2008-08-19
- 2008
- Engelska.
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Ingår i: Proceedings of the National Academy of Sciences of the United States of America. - Washington, DC : National Academy of Sciences. - 0027-8424 .- 1091-6490. ; 105:33, s. 11754-11759
- Relaterad länk:
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http://www.pnas.org/...
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https://urn.kb.se/re...
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https://doi.org/10.1...
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Abstract
Ämnesord
Stäng
- How the crowded environment inside cells affects the structures of proteins with aspherical shapes is a vital question because many proteins and protein–protein complexes in vivo adopt anisotropic shapes. Here we address this question by combining computational and experimental studies of a football-shaped protein (i.e., Borrelia burgdorferi VlsE) in crowded, cell-like conditions. The results show that macromolecular crowding affects protein-folding dynamics as well as overall protein shape. In crowded milieus, distinct conformational changes in VlsE are accompanied by secondary structure alterations that lead to exposure of a hidden antigenic region. Our work demonstrates the malleability of “native” proteins and implies that crowding-induced shape changes may be important for protein function and malfunction in vivo.
Nyckelord
- energy landscape theory
- excluded volume effect
- Lyme disease
- macromolecular crowding
- off-lattice model
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