Evidence for a protein transported through the secretory pathway en route to the higher plant chloroplast

• In contrast to animal and fungal cells, green plant cells contain one or multiple chloroplasts, the organelle(s) in which photosynthetic reactions take place. Chloroplasts are believed to have originated from an endosymbiotic event and contain DNA that codes for some of their proteins. Most chloroplast proteins are encoded by the nuclear genome and imported with the help of sorting signals that are intrinsic parts of the polypeptides. Here, we show that a chloroplast-located protein in higher plants takes an alternative route through the secretory pathway, and becomes N-glycosylated before entering the chloroplast.

Ämnesord

NATURVETENSKAP  -- Biologi -- Biokemi och molekylärbiologi (hsv//swe)

NATURAL SCIENCES  -- Biological Sciences -- Biochemistry and Molecular Biology (hsv//eng)

NATURVETENSKAP  -- Biologi -- Botanik (hsv//swe)

NATURAL SCIENCES  -- Biological Sciences -- Botany (hsv//eng)

Nyckelord

Arabidopsis/metabolism/*ultrastructure

Arabidopsis Proteins/metabolism

Carbonic Anhydrases/genetics

Chloroplasts/*metabolism

Cytoplasmic Vesicles/metabolism

Glycosylation

Green Fluorescent Proteins/genetics

Microscopy; Fluorescence

Protein Sorting Signals

Protein Transport

Transfection

Publikations- och innehållstyp

ref (ämneskategori)

art (ämneskategori)