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Structural basis fo...
Structural basis for catalytically restrictive dynamics of a high-energy enzyme state
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- Kovermann, Michael (författare)
- Umeå universitet,Kemiska institutionen
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- Ådén, Jörgen (författare)
- Umeå universitet,Kemiska institutionen
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- Grundström, Christin (författare)
- Umeå universitet,Kemiska institutionen
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- Sauer-Eriksson, A. Elisabeth (författare)
- Umeå universitet,Kemiska institutionen
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- Sauer, Uwe H (författare)
- Umeå universitet,Kemiska institutionen
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- Wolf-Watz, Magnus (författare)
- Umeå universitet,Kemiska institutionen
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(creator_code:org_t)
- 2015-07-03
- 2015
- Engelska.
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Ingår i: Nature Communications. - : Macmillan Publishers Ltd.. - 2041-1723. ; 6
- Relaterad länk:
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https://doi.org/10.1...
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https://umu.diva-por... (primary) (Raw object)
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https://www.nature.c...
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https://urn.kb.se/re...
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https://doi.org/10.1...
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Abstract
Ämnesord
Stäng
- An emerging paradigm in enzymology is that transient high-energy structural states play crucial roles in enzymatic reaction cycles. Generally, these high-energy or ‘invisible’ states cannot be studied directly at atomic resolution using existing structural and spectroscopic techniques owing to their low populations or short residence times. Here we report the direct NMR-based detection of the molecular topology and conformational dynamics of a catalytically indispensable high-energy state of an adenylate kinase variant. On the basis of matching energy barriers for conformational dynamics and catalytic turnover, it was found that the enzyme’s catalytic activity is governed by its dynamic interconversion between the high-energy state and a ground state structure that was determined by X-ray crystallography. Our results show that it is possible to rationally tune enzymes’ conformational dynamics and hence their catalytic power—a key aspect in rational design of enzymes catalysing novel reactions.
Ämnesord
- NATURVETENSKAP -- Kemi (hsv//swe)
- NATURAL SCIENCES -- Chemical Sciences (hsv//eng)
Nyckelord
- Biological sciences
- Biophysics
- Biochemistry
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
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