Sökning: onr:"swepub:oai:DiVA.org:umu-106966" >
MicroRNA-519c suppr...
MicroRNA-519c suppresses hypoxia-inducible factor-1alpha expression and tumor angiogenesis.
-
Cha, Shih-Ting (författare)
-
Chen, Pai-Sheng (författare)
-
- Johansson, Gunnar (författare)
- 1Laboratory of Molecular and Cellular Toxicology, Institute of Toxicology, College of Medicine, National Taiwan University
-
visa fler...
-
Chu, Chia-Yu (författare)
-
Wang, Ming-Yang (författare)
-
Jeng, Yung-Ming (författare)
-
Yu, Sung-Liang (författare)
-
Chen, Jin-Shing (författare)
-
Chang, King-Jen (författare)
-
Jee, Shiou-Hwa (författare)
-
Tan, Ching-Ting (författare)
-
Lin, Ming-Tsan (författare)
-
Kuo, Min-Liang (författare)
-
visa färre...
-
(creator_code:org_t)
- American Association for Cancer Research, 2010
- 2010
- Engelska.
-
Ingår i: Cancer Research. - : American Association for Cancer Research. - 0008-5472 .- 1538-7445. ; 70:7, s. 2675-2685
- Relaterad länk:
-
https://urn.kb.se/re...
-
visa fler...
-
https://doi.org/10.1...
-
visa färre...
Abstract
Ämnesord
Stäng
- Hypoxia-inducible factor-1alpha (HIF-1alpha) is widely considered to be one of the key regulators of tumor angiogenesis. The upstream regulation is complex and involves several growth factors, cytokines, and hypoxia. Herein, we have identified miR-519c as a hypoxia-independent regulator of HIF-1alpha, acting through direct binding to the HIF-1alpha 3' untranslated region and leading to reduced tumor angiogenesis. Overexpression of miR-519c resulted in a significant decrease of HIF-1alpha protein levels and reduced the tube formation of human umbilical vein endothelial cells; similarly, antagomir inhibition of miR-519c increased the level of HIF-1alpha protein and enhanced angiogenic activity, suggesting an important role of miR-519c in HIF-1alpha-mediated angiogenesis. Consistent with the overexpression of miR-519c in cancer patients with better prognosis, mice injected with miR-519c-overexpressing cells exhibited dramatically reduced HIF-1alpha levels, followed by suppressed tumor angiogenesis, growth, and metastasis. In addition, we found that hepatocyte growth factor (HGF), a known HIF-1alpha inducer, reduced the miR-519c levels through an Akt-dependent pathway. This regulation was posttranscriptional and may be mediated by suppression of miR-519c maturation. Taken together, our findings provide the first evidence that miR-519c is a pivotal regulator of tumor angiogenesis and that microenvironmental HGF contributes to regulating miR-519c biogenesis in cancer cells.
Ämnesord
- NATURVETENSKAP -- Biologi -- Cellbiologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Cell Biology (hsv//eng)
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
Hitta via bibliotek
Till lärosätets databas
- Av författaren/redakt...
-
Cha, Shih-Ting
-
Chen, Pai-Sheng
-
Johansson, Gunna ...
-
Chu, Chia-Yu
-
Wang, Ming-Yang
-
Jeng, Yung-Ming
-
visa fler...
-
Yu, Sung-Liang
-
Chen, Jin-Shing
-
Chang, King-Jen
-
Jee, Shiou-Hwa
-
Tan, Ching-Ting
-
Lin, Ming-Tsan
-
Kuo, Min-Liang
-
visa färre...
- Om ämnet
-
- NATURVETENSKAP
-
NATURVETENSKAP
-
och Biologi
-
och Cellbiologi
- Artiklar i publikationen
-
Cancer Research
- Av lärosätet
-
Umeå universitet