Safranin O reduces loss of glycosaminoglycans from bovine articular cartilage during histological specimen preparation.

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Fältnamn	Indikatorer	Metadata
000		04477naa a2200505 4500
001		oai:DiVA.org:umu-108052
003		SwePub
008		150902s1996 \| \|\|\|\|\|\|\|\|\|\|\|000 \|\|eng\|
024	7	a https://urn.kb.se/resolve?urn=urn:nbn:se:umu:diva-1080522 URI
040		a (SwePub)umu
041		a engb engb -1
042		9 SwePub
072	7	a ref2 swepub-contenttype
072	7	a art2 swepub-publicationtype
100	1	a Király, Kariu Department of Anatomy, University o Kuopio, Kuopio, Finland4 aut
245	1 0	a Safranin O reduces loss of glycosaminoglycans from bovine articular cartilage during histological specimen preparation.
264	1	b Chapman & Hill,c 1996
338		a print2 rdacarrier
520		a The ability of Safranin O, added to fixation and decalcification solutions, to prevent the escape of glycosaminoglycans (GAGs) from small cartilage tissue blocks during histological processing of cartilage has been studied. GAGs in the fixatives and decalcifying solutions used and those remaining in the 1 mm3 cubes of cartilage were assayed biochemically. The quantity of GAGs remaining in the cartilage cubes were determined from Safranin O-stained sections using videomicroscopy or microspectrophotometry. A quantity (10.6%) of GAGs were lost during a conventional 4% buffered formaldehyde fixation (48 h) and a subsequent decalcification in 10% EDTA (12 days) at 4 degrees C. Roughly one-quarter of the total GAG loss occurred during the 48 h fixation, and three-quarters during the 12 days of decalcification. Inclusion of 4% formaldehyde in the decalcification fluid decreased the loss of GAGs to 6.2%. The presence of 0.5% Safranin O in the fixative reduced this loss to 3.4%. When 0.5% Safranin O was included in the fixative and 4% formaldehyde in the decalcification solution, Safranin O staining of the histological sections increased on average by 13.5%. After fixation in the presence of 0.5% Safranin O, there was no difference in the staining intensities when decalcification was carried out in the presence of either Safranin O or formaldehyde, or both. It took 24 h for Safranin O to penetrate into the deep zone of articular cartilage, warranting a fixation period of at least this long. In conclusion, the addition of Safranin O to the fixative and either Safranin O or formaldehyde in the following decalcification fluid, markedly reduces the loss of GAGs from small articular cartilage explants during histological processing. However, for immunohistochemical studies, Safranin O cannot be included in the processing solutions, because it may interfere.
520		a
650	7	a MEDICIN OCH HÄLSOVETENSKAPx Medicinska och farmaceutiska grundvetenskaperx Cell- och molekylärbiologi0 (SwePub)301082 hsv//swe
650	7	a MEDICAL AND HEALTH SCIENCESx Basic Medicinex Cell and Molecular Biology0 (SwePub)301082 hsv//eng
650	7	a NATURVETENSKAPx Biologix Biokemi och molekylärbiologi0 (SwePub)106022 hsv//swe
650	7	a NATURAL SCIENCESx Biological Sciencesx Biochemistry and Molecular Biology0 (SwePub)106022 hsv//eng
650	7	a MEDICIN OCH HÄLSOVETENSKAPx Klinisk medicinx Ortopedi0 (SwePub)302112 hsv//swe
650	7	a MEDICAL AND HEALTH SCIENCESx Clinical Medicinex Orthopaedics0 (SwePub)302112 hsv//eng
653		a Articular cartilage
653		a proteoglycans
653		a histology
653		a sample preparation
653		a biokemi
653		a Biochemistry
653		a cell research
653		a cellforskning
653		a ortopedi
653		a Orthopaedics
700	1	a Lammi, Mikko,d 1961-u Department of Anatomy, University o Kuopio, Kuopio, Finland,Chondrogenic and Osteogenic Differentiation Group4 aut0 (Swepub:umu)mila0077
700	1	a Arokoski, Jariu Department of Anatomy, University o Kuopio, Kuopio, Finland4 aut
700	1	a Lapveteläinen, Tuomou Department of Anatomy, University o Kuopio, Kuopio, Finland4 aut
700	1	a Tammi, Markkuu Department of Anatomy, University o Kuopio, Kuopio, Finland4 aut
700	1	a Helminen, Heikkiu Department of Anatomy, University o Kuopio, Kuopio, Finland4 aut
700	1	a Kiviranta, Ilkka4 aut
710	2	a Department of Anatomy, University o Kuopio, Kuopio, Finlandb Chondrogenic and Osteogenic Differentiation Group4 org
773	0	t The Histochemical Journald : Chapman & Hillg 28:2, s. 99-107q 28:2<99-107x 0018-2214x 1573-6865
856	4 8	u https://urn.kb.se/resolve?urn=urn:nbn:se:umu:diva-108052

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Av författaren/redakt...: Király, Kari; Lammi, Mikko, 19 ...; Arokoski, Jari; Lapveteläinen, T ...; Tammi, Markku; Helminen, Heikki; visa fler...; Kiviranta, Ilkka; visa färre...

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