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Effects of controll...
Effects of controlled diesel exhaust exposure on apoptosis and proliferation markers in bronchial epithelium : an in vivo bronchoscopy study on asthmatics, rhinitics and healthy subjects
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- Behndig, Annelie F. (författare)
- Umeå universitet,Lungmedicin
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Shanmuganathan, Karthika (författare)
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Whitmarsh, Laura (författare)
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- Stenfors, Nikolai (författare)
- Umeå universitet,Medicin,Östersund Research Unit, Umeå University.
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Brown, Joanna L. (författare)
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Frew, Anthony J. (författare)
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Kelly, Frank J. (författare)
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Mudway, Ian S. (författare)
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- Sandström, Thomas (författare)
- Umeå universitet,Lungmedicin
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Wilson, Susan J. (författare)
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(creator_code:org_t)
- 2015-08-25
- 2015
- Engelska.
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Ingår i: BMC Pulmonary Medicine. - : Springer Science and Business Media LLC. - 1471-2466. ; 15
- Relaterad länk:
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https://doi.org/10.1...
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https://umu.diva-por... (primary) (Raw object)
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https://bmcpulmmed.b...
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https://urn.kb.se/re...
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https://doi.org/10.1...
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Abstract
Ämnesord
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- Background: Epidemiological evidence demonstrates that exposure to traffic-derived pollution worsens respiratory symptoms in asthmatics, but controlled human exposure studies have failed to provide a mechanism for this effect. Here we investigated whether diesel exhaust (DE) would induce apoptosis or proliferation in the bronchial epithelium in vivo and thus contribute to respiratory symptoms.Methods: Moderate (n = 16) and mild (n = 16) asthmatics, atopic non-asthmatic controls (rhinitics) (n = 13) and healthy controls (n = 21) were exposed to filtered air or DE (100 μg/m 3 ) for 2 h, on two separate occasions. Bronchial biopsies were taken 18 h post-exposure and immunohistochemically analysed for pro-apoptotic and anti-apoptotic proteins (Bad, Bak, p85 PARP, Fas, Bcl-2) and a marker of proliferation (Ki67). Positive staining was assessed within the epithelium using computerized image analysis.Results: No evidence of epithelial apoptosis or proliferation was observed in healthy, allergic or asthmatic airways following DE challenge.Conclusion: In the present study, we investigated whether DE exposure would affect markers of proliferation and apoptosis in the bronchial epithelium of asthmatics, rhinitics and healthy controls, providing a mechanistic basis for the reported increased airway sensitivity in asthmatics to air pollutants. In this first in vivo exposure investigation, we found no evidence of diesel exhaust-induced effects on these processes in the subject groups investigated.
Ämnesord
- MEDICIN OCH HÄLSOVETENSKAP -- Klinisk medicin -- Lungmedicin och allergi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Clinical Medicine -- Respiratory Medicine and Allergy (hsv//eng)
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
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Behndig, Annelie ...
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Shanmuganathan, ...
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Whitmarsh, Laura
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Stenfors, Nikola ...
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Brown, Joanna L.
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Frew, Anthony J.
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visa fler...
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Kelly, Frank J.
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Mudway, Ian S.
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Sandström, Thoma ...
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Wilson, Susan J.
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visa färre...
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Umeå universitet