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Construction of Aer...
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Schwenteit, J. M.
(författare)
Construction of Aeromonas salmonicida subsp. achromogenes AsaP1-toxoid strains and study of their ability to induce immunity in Arctic char, Salvelinus alpinus L.
- Artikel/kapitelEngelska2015
Förlag, utgivningsår, omfång ...
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2014-10-01
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Wiley,2015
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printrdacarrier
Nummerbeteckningar
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LIBRIS-ID:oai:DiVA.org:umu-109361
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https://urn.kb.se/resolve?urn=urn:nbn:se:umu:diva-109361URI
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https://doi.org/10.1111/jfd.12303DOI
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Språk:engelska
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Sammanfattning på:engelska
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Ämneskategori:ref swepub-contenttype
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Ämneskategori:art swepub-publicationtype
Anmärkningar
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The metalloendopeptidase AsaP1 is one of the major extracellular virulence factors of A. salmonicida subsp. achromogenes, expressed as a 37-kDa pre-pro-peptide and processed to a 19-kDa active peptide. The aim of this study was to construct mutant strains secreting an AsaP1-toxoid instead of AsaP1-wt, to study virulence of these strains and to test the potency of the AsaP1-toxoid bacterin and the recombinant AsaP1-toxoids to induce protective immunity in Arctic char. Two A. salmonicida mutants were constructed that secrete either AsaP1(E294A) or AsaP1(Y309F). The secreted AsaP1(Y309F)-toxoid had weak caseinolytic activity and was processed to the 19-kDa peptide, whereas the AsaP1(E294A)-toxoid was found as a 37-kDa pre-pro-peptide suggesting that AsaP1 is auto-catalytically processed. The LD50 of the AsaP1(Y309F)-toxoid mutant in Arctic char was significantly higher than that of the corresponding wt strain, and LD50 of the AsaP1(E294A)-toxoid mutant was comparable with that of an AsaP1-deficient strain. Bacterin based on AsaP1(Y309F)-toxoid mutant provided significant protection, comparable with that induced by a commercial polyvalent furunculosis vaccine. Detoxification of AsaP1 is very hard, expensive and time consuming. Therefore, an AsaP1-toxoid-secreting mutant is more suitable than the respective wt strain for production of fish bacterins aimed to protect against atypical furunculosis.
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Biuppslag (personer, institutioner, konferenser, titlar ...)
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Weber, BarbaraUmeå universitet,Umeå Centre for Microbial Research (UCMR)(Swepub:umu)bawe0002
(författare)
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Milton, Debra L.Umeå universitet,Institutionen för molekylärbiologi (Medicinska fakulteten),Southern Research Institute, Birmingham, AL, USA,Grupp Debra Milton(Swepub:umu)demi0001
(författare)
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Bornscheuer, U. T.
(författare)
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Gudmundsdottir, B. K.
(författare)
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Umeå universitetUmeå Centre for Microbial Research (UCMR)
(creator_code:org_t)
Sammanhörande titlar
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Ingår i:Journal of Fish Diseases: Wiley38:10, s. 891-9000140-77751365-2761
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