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Comparative study o...
Comparative study of the enzymatic convertibility of glycerol- and dilute acid-pretreated sugarcane bagasse using Penicillium- and Trichoderma-based cellulase preparations
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- Martin, Carlos (författare)
- Umeå universitet,Kemiska institutionen,A.N Bach Institute of Biochemistry, Russian Academy of Sciences, Moscow 119071, Russia; Thünen Institute for Wood Research, 21031 Hamburg, Germany
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Volkov, Pavel V. (författare)
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Rozhkova, Aleksandra M. (författare)
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Puls, Juergen (författare)
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Sinitsyna, Arkady P. (författare)
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(creator_code:org_t)
- Elsevier BV, 2015
- 2015
- Engelska.
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Ingår i: Industrial crops and products (Print). - : Elsevier BV. - 0926-6690 .- 1872-633X. ; 77, s. 382-390
- Relaterad länk:
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https://urn.kb.se/re...
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https://doi.org/10.1...
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Abstract
Ämnesord
Stäng
- Three enzyme preparations based on the cellulase complex of Penicillium verruculosum and three Trichoderma reesei-based enzyme cocktails were used for evaluating the enzymatic convertibility of cellulose contained in glycerol- and sulfuric acid-pretreated bagasse. The hydrolysis was initially monitored with a micro-scale method using 2 mL of reaction mixture containing 50 g/L of pretreated solids, and at an enzyme load of 10 mg proteinig cellulose. The results were further validated at a higher scale in a setup consisting of 20 mL of reaction mixture with a substrate concentration of 100 g/L. For all the cellulase preparations, and regardless of the experiment scale, glycerol-pretreated bagasse displayed better enzymatic convertibility than acid-pretreated bagasse. It was observed that when the enzyme load is increased from 2 to 10 mg/g, the cellulose conversion is improved but the specific hydrolysis rate is only marginally affected. Although the Trichoderma-based commercial cocktail CC-3 led to higher hydrolysis rates and conversions than all the other enzyme preparations, the Penicillium-based cellulases, especially PV-Xyl PCA and PV-Hist BGL, also showed good potential. PV-Xyl PCA was relatively effective for hydrolysing acid-pretreated bagasse, and PV-Hist BGL displayed reasonable performance in the hydrolysis in absence of exogenous beta-glucosidase.
Ämnesord
- NATURVETENSKAP -- Kemi (hsv//swe)
- NATURAL SCIENCES -- Chemical Sciences (hsv//eng)
Nyckelord
- Cellulases
- Enzymatic hydrolysis
- Pretreatment
- Sugarcane bagasse
- Penicillium
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
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