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Functional analysis...
Functional analysis of the PsbP-like protein (sll1418) in Synechocystis sp PCC 6803
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- Ishikawa, Yasuo (författare)
- Umeå universitet,Kemiska institutionen
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- Schröder, Wolfgang P (författare)
- Umeå universitet,Kemiska institutionen
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- Funk, Christiane (författare)
- Umeå universitet,Kemiska institutionen
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(creator_code:org_t)
- Dordrecht : Springer, 2005
- 2005
- Engelska.
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Ingår i: Photosynthesis Research. - Dordrecht : Springer. - 0166-8595 .- 1573-5079. ; 84:1-3, s. 257-262
- Relaterad länk:
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https://urn.kb.se/re...
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https://doi.org/10.1...
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Abstract
Ämnesord
Stäng
- A recent proteomic analysis of the thylakoid lumen of Arabidopsis thaliana revealed the presence of several PsbP-like proteins, and a homologue to this gene family was detected in the genome of the cyanobacterium Synechocystis sp. PCC 6803 (Schubert M, Petersson UA, Haas BJ, Funk C, Schroder WP, Kieselbach T (2002) J Biol Chem 277, 8354-8365). Using a peptide-directed antibody against this cyanobacterial PsbP-like protein (sll1418) we could show that it was localized in the thylakoid membrane and associated with Photosystem II. While salt washes did not remove the PsbP-like protein from the thylakoid membrane, it was partially lost during the detergent-based isolation of PSII membrane fractions. In total cell extracts this protein is present in the same amount as the extrinsic PsbO protein. We did not see any significant functional difference between the wild-type and a PsbP-like insertion mutant.
Ämnesord
- LANTBRUKSVETENSKAPER -- Bioteknologi med applikationer på växter och djur -- Växtbioteknologi (hsv//swe)
- AGRICULTURAL SCIENCES -- Agricultural Biotechnology -- Plant Biotechnology (hsv//eng)
Nyckelord
- cyanobacteria
- extrinsic proteins
- oxygen-evolving complex
- Photosystem II
- PsbP
- Synechocystis sp PCC 6803
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
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