Sökning: onr:"swepub:oai:DiVA.org:umu-12695" >
Vasoactive intestin...
Vasoactive intestinal polypeptide and pituitary adenylate cyclase activating polypeptide : effects on insulin release in isolated mouse islets in relation to metabolic status and age.
-
- Persson-Sjögren, Solveig (författare)
- Umeå universitet,Histologi med cellbiologi,Biomedicinsk laboratorievetenskap
-
- Forsgren, Sture (författare)
- Umeå universitet,Anatomi
-
- Lindström, Per (författare)
- Umeå universitet,Histologi med cellbiologi
-
(creator_code:org_t)
- Elsevier BV, 2006
- 2006
- Engelska.
-
Ingår i: Neuropeptides. - : Elsevier BV. - 0143-4179. ; 40:4, s. 283-90
- Relaterad länk:
-
http://www.ncbi.nlm....
-
visa fler...
-
https://urn.kb.se/re...
-
https://doi.org/10.1...
-
visa färre...
Abstract
Ämnesord
Stäng
- Obesity and development of the metabolic syndrome is related to an increased parasympathetic tone and hyperinsulinemia. We have now studied the effects of age and metabolic status on glucose-induced insulin release stimulated by the neuropeptides vasoactive intestinal polypeptide (VIP; 10 nM) and pituitary adenylate cyclase activating polypeptide (PACAP; 10 nM), that are constituents of the parasympathetic nerves in the islets, and the cholinergic agonists acetylcholine (ACh; 10 microM) and carbachol (10 microM), in isolated islets from female obese ob/ob mice and lean mice. Both VIP and PACAP enhanced insulin secretion in islets from 4-week-old hyperglycemic ob/ob mice. VIP did not increase 11.1 mM glucose-induced insulin release in islets from 4-week-old lean normoglycemic mice and neither did PACAP in the absence of bicarbonate. The neuropeptides increased insulin release in islets from 9 to 10-month-old mice but VIP and PACAP had no effect in islets from very old mice. ACh had no effect in islets from 9 to 10-months and older ob/ob mice in the absence of bicarbonate. The combination of VIP and cholinergic agonists had an additive effect in islets from ob/ob mice, and PACAP combined with carbachol potentiated insulin release in islets from 4-week-old lean mice. VIP increased early phase insulin release in perifused islets from young mice. A higher concentration of theophylline was needed to potentiate glucose-induced insulin release in islets from young lean mice than in islets from old lean mice and ob/ob mice. The present results demonstrate age-related dynamics in the effects of neuropeptides affecting cAMP in pancreatic islets. We suggest that VIP and PACAP contribute to the developing metabolic syndrome in ob/ob mice by aggravating hyperinsulinemia.
Nyckelord
- Aging/*physiology
- Animals
- Carbachol/pharmacology
- Energy Metabolism
- Female
- Insulin/*metabolism
- Islets of Langerhans/*drug effects/metabolism
- Mice
- Mice; Obese
- Muscarinic Agonists/pharmacology
- Pituitary Adenylate Cyclase-Activating Polypeptide/*pharmacology
- Tissue Culture Techniques
- Vasoactive Intestinal Peptide/*pharmacology
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
Hitta via bibliotek
Till lärosätets databas