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Recombinant express...
Recombinant expression of Mimivirus L725 ORFan gene product
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Sobhy, H. (författare)
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Gotthard, G. (författare)
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Chabriere, E. (författare)
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Raoult, D. (författare)
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- Colson, P. (författare)
- Umeå universitet,Institutionen för molekylärbiologi (Medicinska fakulteten),Aix Marseille Univ, Marseille, France ; Ctr Hosp Univ Timone, AP HM, IHU Mediterranee Infect, Pole Malad Infect & Tropicales Clin & Biol,Federa, Marseille, France
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(creator_code:org_t)
- AEPRESS SRO, 2017
- 2017
- Engelska.
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Ingår i: Acta virologica. - : AEPRESS SRO. - 0001-723X .- 1336-2305. ; 61:1, s. 123-126
- Relaterad länk:
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http://www.elis.sk/d...
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https://urn.kb.se/re...
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https://doi.org/10.4...
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Abstract
Ämnesord
Stäng
- Mimivirus was the first discovered amoebal giant virus. The Mimivirus virions are covered by a dense layer of approximately 130 nm-long fibers, the length and shape of which diverge from those of other viruses. Here, we aimed at expressing the L725 protein to further confirm and study its role as a fiber-associated protein. We report Escherichia coli expression of the L725 protein, which is encoded by a Mimivirus ORFan, was previously identified by proteomics in purified viral fibers and demonstrated to be a fiber-associated protein by RNA-silencing experiments. The expressed protein was recognized by anti-Mimivirus fiber or anti-Mimivirus L725 polyclonal antibodies. This study is the only expression, to our knowledge, of a product from a Mimiviral ORFan gene.
Ämnesord
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinska och farmaceutiska grundvetenskaper -- Cell- och molekylärbiologi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Basic Medicine -- Cell and Molecular Biology (hsv//eng)
Nyckelord
- Mimivirus
- giant virus
- fiber
- ORFan
- gene expression
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
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