Quantification of bound bicarbonate in photosystem II

In this study, we presented a new approach for quantification of bicarbonate (HCO3-) molecules bound to PSII. Our method, which is based on a combination of membrane-inlet mass spectrometry (MIMS) and O-18-labelling, excludes the possibility of "non-accounted" HCO3- by avoiding (1) the employment of formate for removal of HCO3- from PSII, and (2) the extremely low concentrations of HCO3-/CO2 during online MIMS measurements. By equilibration of PSII sample to ambient CO2 concentration of dissolved CO2/HCO3-, the method ensures that all physiological binding sites are saturated before analysis. With this approach, we determined that in spinach PSII membrane fragments 1.1 +/- 0.1 HCO3- are bound per PSII reaction center, while none was bound to isolated PsbO protein. Our present results confirmed that PSII binds one HCO3- molecule as ligand to the non-heme iron of PSII, while unbound HCO3- optimizes the water-splitting reactions by acting as a mobile proton shuttle.

Shevela, DmitryUmeå universitet,Kemiska institutionen(Swepub:umu)dmsh0004 (författare)
Klimov, V. V. (författare)
Messinger, JohannesUmeå universitet,Kemiska institutionen,Department of Chemistry, Molecular Biomimetics, Ångström Laboratory, Uppsala University, Uppsala, Sweden(Swepub:umu)jome0007 (författare)
Umeå universitetKemiska institutionen (creator_code:org_t)

Ingår i:Photosynthetica (Praha): Institute of Experimental Botany56:1, s. 210-2160300-36041573-9058

Av författaren/redakt...: Tikhonov, K.; Shevela, Dmitry; Klimov, V. V.; Messinger, Johan ...

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