Sökning: onr:"swepub:oai:DiVA.org:umu-14771" > Structural Bases of...
Fältnamn | Indikatorer | Metadata |
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000 | 02931naa a2200361 4500 | |
001 | oai:DiVA.org:umu-14771 | |
003 | SwePub | |
008 | 070626s2004 | |||||||||||000 ||eng| | |
024 | 7 | a https://urn.kb.se/resolve?urn=urn:nbn:se:umu:diva-147712 URI |
024 | 7 | a https://doi.org/10.1016/j.jmb.2004.10.0102 DOI |
040 | a (SwePub)umu | |
041 | a engb eng | |
042 | 9 SwePub | |
072 | 7 | a ref2 swepub-contenttype |
072 | 7 | a art2 swepub-publicationtype |
100 | 1 | a Lobov, Sergeiu Umeå universitet,Institutionen för medicinsk kemi och biofysik4 aut |
245 | 1 0 | a Structural Bases of the Redox-dependent Conformational Switch in the Serpin PAI-2 |
264 | 1 | b Elsevier BV,c 2004 |
338 | a print2 rdacarrier | |
520 | a Depending on the redox-status, the serpin plasminogen activator inhibitor type 2 (PAI-2) can exist in either a stable monomeric or polymerogenic form. The latter form, which spontaneously forms loop-sheet polymers, has an open β-sheet A and is stabilized by a disulfide bond between C79 (in the CD-loop) and C161 (at the bottom of PAI-2). Reduction of this bond results in a closing of the β-sheet A and converts PAI-2 to a stable monomeric form. Here we show that the stable monomeric and polymerogenic forms of PAI-2 are fully interconvertible, depending on redox-status of the environment. Our intramolecular distance measurements indicate that the CD-loop folds mainly on one side of the stable monomeric form of the inhibitor. However, the loop can translocate about 54 Å to the bottom of PAI-2 so that the C79–C161 disulfide bond can form under oxidizing conditions. We show also that the redox-active C79 can form a disulfide-link to the matrix protein vitronectin, suggesting that vitronectin can stabilize active PAI-2 in extracellular compartments. PAI-2 is therefore a rare example of a redox-sensitive protein for which the activity and polymerization ability are regulated by reversible disulfide bond formation leading to major translocation of a loop and significant conformational changes in the molecule. | |
653 | a PAI-2 | |
653 | a redox | |
653 | a serpin | |
653 | a vitronectin | |
653 | a homo-transfer | |
700 | 1 | a Wilczynska, Malgorzatau Umeå universitet,Institutionen för medicinsk kemi och biofysik4 aut0 (Swepub:umu)mawi0006 |
700 | 1 | a Bergström, Fredriku Umeå universitet,Kemiska institutionen4 aut |
700 | 1 | a Johansson, Lennart B-Åu Umeå universitet,Kemiska institutionen4 aut0 (Swepub:umu)lejo0007 |
700 | 1 | a Ny, Toru Umeå universitet,Institutionen för medicinsk kemi och biofysik4 aut0 (Swepub:umu)tony0001 |
710 | 2 | a Umeå universitetb Institutionen för medicinsk kemi och biofysik4 org |
773 | 0 | t Journal of Molecular Biologyd : Elsevier BVg 344:5, s. 1359-68q 344:5<1359-68x 0022-2836 |
856 | 4 8 | u https://urn.kb.se/resolve?urn=urn:nbn:se:umu:diva-14771 |
856 | 4 8 | u https://doi.org/10.1016/j.jmb.2004.10.010 |
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