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Object plane detect...
Object plane detection and phase retrieval from single-shot holograms using multi-wavelength in-line holography
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- Zhang, Hanqing (författare)
- Umeå universitet,Institutionen för fysik,Biophysics & Biophotonics group
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- Stangner, Tim (författare)
- Umeå universitet,Institutionen för fysik
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- Wiklund, Krister (författare)
- Umeå universitet,Institutionen för fysik
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visa fler...
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- Andersson, Magnus (författare)
- Umeå universitet,Institutionen för fysik,Umeå Centre for Microbial Research (UCMR)
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visa färre...
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(creator_code:org_t)
- Engelska.
- Relaterad länk:
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https://urn.kb.se/re...
Abstract
Ämnesord
Stäng
- Phase retrieval and the twin-image problem in digital in-line holographic microscopy can be resolvedby iterative reconstruction routines. However, recovering the phase properties of an object in a hologramneeds an object plane to be chosen correctly for reconstruction. In this work, we present a novelmulti-wavelength Gerchberg-Saxton algorithm to determine the object plane using single-shot hologramsrecorded with multiple wavelengths in an in-line holographic microscope. For micro-sized objects, weverify the object positioning capabilities of the method for various shapes and derive the phase informationusing synthetic and experimental data. Experimentally, we built a compact digital in-line holographicmicroscopy setup around a standard optical microscope with a regular RGB-CCD camera andacquire holograms of micro-spheres, E. coli and red blood cells, that are illuminated using three lasersoperating at 491nm, 532nm and 633nm, respectively. We demonstrate that our method provides accurateobject plane detection and phase retrieval under noisy conditions, e.g., using low-contrast hologramswithout background normalization. This method allows for automatic positioning and phase retrievalsuitable for holographic particle velocimetry, and object tracking in biophysical or colloidal research.
Ämnesord
- NATURVETENSKAP -- Biologi -- Biofysik (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Biophysics (hsv//eng)
Nyckelord
- digital holographic microscopy
- image reconstruction
- multiple wavelengths
- fysik
- Physics
Publikations- och innehållstyp
- vet (ämneskategori)
- ovr (ämneskategori)