Sökning: onr:"swepub:oai:DiVA.org:umu-156612" >
A standardized prot...
-
Claeson, Anna-Sara,1974-Umeå universitet,Institutionen för psykologi
(författare)
A standardized protocol for comparable analysis of GSH/GSSG by UHPLC-ESI-MSMS for human plasma
- Artikel/kapitelEngelska2019
Förlag, utgivningsår, omfång ...
-
Elsevier,2019
-
printrdacarrier
Nummerbeteckningar
-
LIBRIS-ID:oai:DiVA.org:umu-156612
-
https://urn.kb.se/resolve?urn=urn:nbn:se:umu:diva-156612URI
-
https://doi.org/10.1016/j.jchromb.2018.11.007DOI
Kompletterande språkuppgifter
-
Språk:engelska
-
Sammanfattning på:engelska
Ingår i deldatabas
Klassifikation
-
Ämneskategori:ref swepub-contenttype
-
Ämneskategori:art swepub-publicationtype
Anmärkningar
-
Variability in the levels of GSH and GSSG in plasma is suggested to derive from inadequate pre-processing methods. The aim of this study was to develop a protocol for comparable and reliable measurements of GSH/GSSG. Venous blood from 8 healthy individuals were collected and divided into 7 different pre-processing procedures. For three of the samples an extraction mixture was added after 0 (baseline), 4 and 8 min and for three of the samples the extraction mixture was added at different times during defrost. A worst case scenario where a sample was left in a cool box during 6 h was also included. The samples were analyzed with UHPLC-ESIMSMS. A large difference in the levels of GSH and GSSG were identified and it was clearly associated with the sample handling procedures. A sample left untreated for 4 min will have significantly reduced amount of GSH. Stability tests showed that the level of GSH was reduced after 3 months in -80 degrees C.
Ämnesord och genrebeteckningar
Biuppslag (personer, institutioner, konferenser, titlar ...)
-
Gouveia-Figueira, Sandra C.Swedish Metabolomics Centre (SMC), Umeå, Sweden(Swepub:umu)sago0010
(författare)
-
Stenlund, HansSwedish Metabolomics Centre (SMC), Umeå, Sweden(Swepub:umu)hasstd97
(författare)
-
Johansson, Annika I.Swedish Metabolomics Centre (SMC), Umeå, Sweden
(författare)
-
Umeå universitetInstitutionen för psykologi
(creator_code:org_t)
Sammanhörande titlar
-
Ingår i:Journal of chromatography. B: Elsevier1104, s. 67-721570-02321873-376X
Internetlänk
Hitta via bibliotek
Till lärosätets databas