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The Medicago CDKC;1...
The Medicago CDKC;1-CYCLINT;1 kinase complex phosphorylates the carboxy-terminal domain of RNA polymerase II and promotes transcription.
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Fülöp, Katalin (författare)
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Pettkó-Szandtner, Aladàr (författare)
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Magyar, Zoltán (författare)
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Miskolczi, Pál (författare)
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Kondorosi, Eva (författare)
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Dudits, Dénes (författare)
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- Bako, Laszlo (författare)
- Umeå universitet,Institutionen för fysiologisk botanik,Umeå Plant Science Centre (UPSC)
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(creator_code:org_t)
- 2005
- 2005
- Engelska.
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Ingår i: Plant Journal. - 0960-7412. ; 42:6, s. 810-20
- Relaterad länk:
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http://www.ncbi.nlm....
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https://urn.kb.se/re...
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https://doi.org/10.1...
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Abstract
Ämnesord
Stäng
- The Ms;CDKC;1 kinase is structurally similar to those cyclin-dependent kinases (CDKs) that are not involved directly in cell cycle regulation. The presence of a PITAIRE motif in Ms;CDKC;1 suggests that it interacts with cyclins different from known PSTAIRE/PPTALRE kinase regulatory subunits. Here we demonstrate that a Medicago CYCLINT (CYCT) protein is a specific interactor of Ms;CDKC;1 and the interaction between these two proteins gives rise to an active kinase complex that localizes to the nucleus and phosphorylates the carboxy-terminal YSPTSPS heptapeptide repeat domain (CTD) of the largest subunit of RNA polymerase II in vitro. Mutation of Ser to Ala at position 5 within the heptapeptide repeat abolishes substrate phosphorylation by the Ms;CDKC;1 kinase complex. Furthermore, our data show that addition of the Medicago CDKC;1-CYCT;1 heterodimer completely restored the transcriptional activity of a HeLa nuclear extract depleted of endogeneous CDK9 kinase complexes. Together, these results indicate that the Medicago CDKC;1-CYCT;1 complex is a positive regulator of transcription in plants and has a role similar to the CDK9/cyclin T complex of human positive transcription elongation factor P-TEFb.
Nyckelord
- Amino Acid Sequence
- Cell Nucleus/metabolism
- Cyclin-Dependent Kinases/metabolism
- Cyclins/metabolism
- Gene Expression Regulation; Plant
- Hela Cells
- Humans
- Medicago/*enzymology
- Molecular Sequence Data
- Multienzyme Complexes/*metabolism
- Plant Proteins/*metabolism
- RNA Polymerase II/*metabolism
- Sequence Alignment
- Sequence Homology; Amino Acid
- Transcription; Genetic
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
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