Sökning: onr:"swepub:oai:DiVA.org:umu-165842" >
Decreased Rate of P...
Decreased Rate of Plasma Arginine Appearance in Murine Malaria May Explain Hypoargininemia in Children With Cerebral Malaria
-
Alkaitis, Matthew S. (författare)
-
Wang, Honghui (författare)
-
Ikeda, Allison K. (författare)
-
visa fler...
-
Rowley, Carol A. (författare)
-
MacCormick, Ian J. C. (författare)
-
Chertow, Jessica H. (författare)
-
- Billker, Oliver (författare)
- Wellcome Trust Sanger Institute, Hinxton, UK
-
Suffredini, Anthony F. (författare)
-
Roberts, David J. (författare)
-
Taylor, Terrie E. (författare)
-
Seydel, Karl B. (författare)
-
Ackerman, Hans C. (författare)
-
visa färre...
-
(creator_code:org_t)
- 2016-12-05
- 2016
- Engelska.
-
Ingår i: Journal of Infectious Diseases. - : Oxford University Press. - 0022-1899 .- 1537-6613. ; 214:12, s. 1840-1849
- Relaterad länk:
-
https://academic.oup...
-
visa fler...
-
https://urn.kb.se/re...
-
https://doi.org/10.1...
-
visa färre...
Abstract
Ämnesord
Stäng
- BACKGROUND: Plasmodium infection depletes arginine, the substrate for nitric oxide synthesis, and impairs endothelium-dependent vasodilation. Increased conversion of arginine to ornithine by parasites or host arginase is a proposed mechanism of arginine depletion.METHODS: We used high-performance liquid chromatography to measure plasma arginine, ornithine, and citrulline levels in Malawian children with cerebral malaria and in mice infected with Plasmodium berghei ANKA with or without the arginase gene. Heavy isotope-labeled tracers measured by quadrupole time-of-flight liquid chromatography-mass spectrometry were used to quantify the in vivo rate of appearance and interconversion of plasma arginine, ornithine, and citrulline in infected mice.RESULTS: Children with cerebral malaria and P. berghei-infected mice demonstrated depletion of plasma arginine, ornithine, and citrulline. Knock out of Plasmodium arginase did not alter arginine depletion in infected mice. Metabolic tracer analysis demonstrated that plasma arginase flux was unchanged by P. berghei infection. Instead, infected mice exhibited decreased rates of plasma arginine, ornithine, and citrulline appearance and decreased conversion of plasma citrulline to arginine. Notably, plasma arginine use by nitric oxide synthase was decreased in infected mice.CONCLUSIONS: Simultaneous arginine and ornithine depletion in malaria parasite-infected children cannot be fully explained by plasma arginase activity. Our mouse model studies suggest that plasma arginine depletion is driven primarily by a decreased rate of appearance.
Ämnesord
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinska och farmaceutiska grundvetenskaper -- Cell- och molekylärbiologi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Basic Medicine -- Cell and Molecular Biology (hsv//eng)
Nyckelord
- metabolism
- host response
- plasmodium
- nitric oxide
- endothelium
- urea cycle
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
Hitta via bibliotek
Till lärosätets databas
- Av författaren/redakt...
-
Alkaitis, Matthe ...
-
Wang, Honghui
-
Ikeda, Allison K ...
-
Rowley, Carol A.
-
MacCormick, Ian ...
-
Chertow, Jessica ...
-
visa fler...
-
Billker, Oliver
-
Suffredini, Anth ...
-
Roberts, David J ...
-
Taylor, Terrie E ...
-
Seydel, Karl B.
-
Ackerman, Hans C ...
-
visa färre...
- Om ämnet
-
- MEDICIN OCH HÄLSOVETENSKAP
-
MEDICIN OCH HÄLS ...
-
och Medicinska och f ...
-
och Cell och molekyl ...
- Artiklar i publikationen
-
Journal of Infec ...
- Av lärosätet
-
Umeå universitet