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Ex Vivo Organoid Cu...
Ex Vivo Organoid Cultures Reveal the Importance of the Tumor Microenvironment for Maintenance of Colorectal Cancer Stem Cells
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- Li, Xingru (författare)
- Umeå universitet,Patologi
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- Larsson, Pär (författare)
- Umeå universitet,Patologi
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- Ljuslinder, Ingrid, 1968- (författare)
- Umeå universitet,Onkologi
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- Öhlund, Daniel, 1979- (författare)
- Umeå universitet,Wallenberg centrum för molekylär medicin vid Umeå universitet (WCMM),Onkologi
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- Myte, Robin (författare)
- Umeå universitet,Onkologi
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- Löfgren Burström, Anna (författare)
- Umeå universitet,Patologi
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- Zingmark, Carl, 1975- (författare)
- Umeå universitet,Patologi
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- Ling, Agnes (författare)
- Umeå universitet,Patologi
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- Edin, Sofia (författare)
- Umeå universitet,Patologi
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- Palmqvist, Richard (författare)
- Umeå universitet,Patologi
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(creator_code:org_t)
- 2020-04-10
- 2020
- Engelska.
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Ingår i: Cancers. - : MDPI. - 2072-6694. ; 12:4
- Relaterad länk:
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https://doi.org/10.3...
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https://umu.diva-por... (primary) (Raw object)
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https://www.mdpi.com...
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https://urn.kb.se/re...
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https://doi.org/10.3...
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Abstract
Ämnesord
Stäng
- Colorectal cancer (CRC) is a heterogeneous disease, with varying clinical presentations and patient prognosis. Different molecular subgroups of CRC should be treated differently and therefore, must be better characterized. Organoid culture has recently been suggested as a good model to reflect the heterogeneous nature of CRC. However, organoid cultures cannot be established from all CRC tumors. The study examines which CRC tumors are more likely to generate organoids and thus benefit from ex vivo organoid drug testing. Long-term organoid cultures from 22 out of 40 CRC tumor specimens were established. It was found that organoid cultures were more difficult to establish from tumors characterized as microsatellite instable (MSI), BRAF-mutated, poorly differentiated and/or of a mucinous type. This suggests that patients with such tumors are less likely to benefit from ex vivo organoid drug testing, but it may also suggest biological difference in tumor growth. RNA sequencing analysis of tumor sections revealed that the in vivo maintenance of these non-organoid-forming tumors depends on factors related to inflammation and pathogen exposure. Furthermore, using TCGA data we could show a trend towards a worse prognosis for patients with organoid-forming tumors, suggesting also clinical differences. Results suggest that organoids are more difficult to establish from tumors characterized as MSI, BRAF-mutated, poorly differentiated and/or of a mucinous type. We further suggest that the maintenance of cell growth of these tumors in vivo may be promoted by immune-related factors and other stromal components within the tumor microenvironment.
Ämnesord
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinska och farmaceutiska grundvetenskaper -- Cell- och molekylärbiologi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Basic Medicine -- Cell and Molecular Biology (hsv//eng)
- MEDICIN OCH HÄLSOVETENSKAP -- Klinisk medicin -- Cancer och onkologi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Clinical Medicine -- Cancer and Oncology (hsv//eng)
Nyckelord
- organoid
- colorectal cancer
- cancer stem cell
- molecular profiling
- tumor microenvironment
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
Hitta via bibliotek
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Cancers
(Sök värdpublikationen i LIBRIS)
Till lärosätets databas
- Av författaren/redakt...
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Li, Xingru
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Larsson, Pär
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Ljuslinder, Ingr ...
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Öhlund, Daniel, ...
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Myte, Robin
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Löfgren Burström ...
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visa fler...
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Zingmark, Carl, ...
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Ling, Agnes
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Edin, Sofia
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Palmqvist, Richa ...
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visa färre...
- Om ämnet
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- MEDICIN OCH HÄLSOVETENSKAP
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MEDICIN OCH HÄLS ...
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och Medicinska och f ...
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och Cell och molekyl ...
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- MEDICIN OCH HÄLSOVETENSKAP
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MEDICIN OCH HÄLS ...
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och Klinisk medicin
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och Cancer och onkol ...
- Artiklar i publikationen
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Cancers
- Av lärosätet
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Umeå universitet