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Immune responses ag...
Immune responses against autologous tumor and human papilloma virus in lymph nodes from patients with penile cancer
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Zhang, Lu (författare)
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- Zirakzadeh, A. Ali (författare)
- Umeå universitet,Urologi och andrologi
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- Rosvall, Jesper (författare)
- Karolinska Institutet
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Hedlund, Mats (författare)
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Hu, Ping-Sheng (författare)
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- Riklund, Katrine, MD, PhD, Professor, 1963- (författare)
- Umeå universitet,Diagnostisk radiologi
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- Sherif, Amir (författare)
- Umeå universitet,Urologi och andrologi
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Winqvist, Ola (författare)
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(creator_code:org_t)
- The Korean Urological Association, 2021
- 2021
- Engelska.
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Ingår i: Investigative and Clinical Urology. - : The Korean Urological Association. - 2466-0493 .- 2466-054X. ; 62
- Relaterad länk:
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https://doi.org/10.4...
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https://umu.diva-por... (primary) (Raw object)
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https://doi.org/10.4...
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https://urn.kb.se/re...
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https://doi.org/10.4...
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http://kipublication...
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Abstract
Ämnesord
Stäng
- PURPOSE: Nearly half of penile cancers are related to human papillomavirus (HPV) infection. Investigations of tumor- and HPV-specific T cell reactivity in regional lymph nodes (LNs) from patients with penile cancer are warranted.MATERIALS AND METHODS: In this study, single-cell suspensions from LNs and peripheral blood from 11 patients with penile cancer were stained with antibodies for lymphocyte markers and analyzed by fluorescence-activated cell sorting (FACS). DNA was extracted from the tumor tissue and HPV status was investigated by PCR.RESULTS: T-cell reactivity against autologous tumor-extract and against the HPV-vaccine Gardasil® was tested by flow-cytometric assay of specific cell-mediated immune response in activated whole blood (FASCIA). CD4+/CD8+ ratios were significantly lower in HPV positive LNs (p<0.05). Immune responses to tumor extract assessed by blast transformation and expansion in vitro, of either CD4+ or CD8+ T-cells, were found in 9 of 13 LNs (69%). 5 of 6 tested patients demonstrated T cell recognition of tumor-associated antigen(s). In HPV-positive patients, dose-dependent T cell responses against L1 (late) HPV proteins (Gardasil vaccine) were demonstrated.CONCLUSIONS: LN-derived T cells from patients with penile cancer recognize tumor antigen(s) and in HPV-positive cases, there is a response against L1 (late) HPV proteins, being constituents of the Gardasil vaccine.
Ämnesord
- MEDICIN OCH HÄLSOVETENSKAP -- Klinisk medicin -- Urologi och njurmedicin (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Clinical Medicine -- Urology and Nephrology (hsv//eng)
Nyckelord
- Adoptive immunity
- Lymph node excision
- Papillomaviridae
- Penile neoplasms
- T-lymphocytes
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
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