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Replication of Salm...
Replication of Salmonella enterica serovar Typhimurium in RAW264.7 Phagocytes Correlates With Hypoxia and Lack of iNOS Expression
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Wrande, Marie (författare)
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- Vestö, Kim (författare)
- Karolinska Institutet
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Banesaru, Speranta Puiac (författare)
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Anwar, Naeem (författare)
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Nordfjell, Johan (författare)
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Liu, Lifeng (författare)
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- McInerney, Gerald M. (författare)
- Karolinska Institutet
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- Rhen, Mikael (författare)
- Umeå universitet,Institutionen för molekylärbiologi (Medicinska fakulteten),Department of Microbiology, Tumor and Cell Biology, Karolinska Institutet, Stockholm, SwedenDepartment of Microbiology, Tumor and Cell Biology, Karolinska Institutet, Stockholm, Sweden
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(creator_code:org_t)
- 2020-11-30
- 2020
- Engelska.
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Ingår i: Frontiers in Cellular and Infection Microbiology. - : Frontiers Media S.A.. - 2235-2988. ; 10
- Relaterad länk:
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https://doi.org/10.3...
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https://umu.diva-por... (primary) (Raw object)
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https://www.frontier...
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https://urn.kb.se/re...
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https://doi.org/10.3...
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http://kipublication...
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Abstract
Ämnesord
Stäng
- Salmonella infection associates with tissue hypoxia, while inducible nitric oxide synthase (iNOS), relying for its activity on molecular oxygen, stands as a central host defence measure in murine salmonellosis. Here, we have detailed hypoxia and iNOS responses of murine macrophage-like RAW264.7 cells upon infection with Salmonella enterica serovar Typhimurium. We noted that only a proportion of the infected RAW264.7 cells became hypoxic or expressed iNOS. Heavily infected cells became hypoxic, while in parallel such cells tended not to express iNOS. While a proportion of the infected RAW264.7 cells revealed shutdown of protein synthesis, this was only detectable after 12 h post infection and after iNOS expression was induced in the cell culture. Our data implicate an intrinsic heterogeneity with regard to hypoxia and iNOS expression in a cell culture-based infection setting.
Ämnesord
- NATURVETENSKAP -- Biologi -- Mikrobiologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Microbiology (hsv//eng)
Nyckelord
- Salmonella enterica
- hypoxia
- iNOS
- macrophages
- bacterial infection
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
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