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Sialic acid as a bi...
Sialic acid as a biomarker studied in breast cancer cell lines in vitro using fluorescent molecularly imprinted polymers
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- El-Schich, Zahra (författare)
- Malmö University,Malmö universitet,Institutionen för biomedicinsk vetenskap (BMV),Biofilms Research Center for Biointerfaces
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- Zhang, Yuecheng (författare)
- Malmö University,Malmö universitet,Institutionen för biomedicinsk vetenskap (BMV),Biofilms Research Center for Biointerfaces
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- Göransson, Tommy (författare)
- Malmö University,Malmö universitet,Institutionen för biomedicinsk vetenskap (BMV)
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- Dizeyi, Nishtman (författare)
- Lund University,Lunds universitet,Molekylärgenetisk reproduktionsmedicin, Malmö,Forskargrupper vid Lunds universitet,Molecular genetic reproductive medicine, Malmö,Lund University Research Groups
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- Persson, Jenny L., Professor (författare)
- Malmö University,Malmö universitet,Umeå universitet,Institutionen för molekylärbiologi (Medicinska fakulteten),Department of Biomedical Sciences, Faculty of Health and Society, Malmö University, Malmö; Biofilms-Research Center for Biointerfaces, Malmö University,Institutionen för biomedicinsk vetenskap (BMV),Biofilms Research Center for Biointerfaces,Umeå University
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- Johansson, Emil, 1985- (författare)
- Umeå universitet,Kemiska institutionen,Umeå Centre for Microbial Research (UCMR),Umeå University
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- Caraballo, Remi (författare)
- Umeå universitet,Kemiska institutionen,Umeå Centre for Microbial Research (UCMR),Umeå University
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- Elofsson, Mikael (författare)
- Umeå universitet,Kemiska institutionen,Umeå Centre for Microbial Research (UCMR),Umeå University
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- Shinde, Sudhirkumar (författare)
- School of Consciousness, Vishwanath Karad Maharashtra Institute of Technology—World Peace University, Kothrud, Pune, India,MAEER's Maharashtra Institute of Technology
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- Sellergren, Börje (författare)
- Malmö University,Malmö universitet,Institutionen för biomedicinsk vetenskap (BMV),Biofilms Research Center for Biointerfaces
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- Gjörloff Wingren, Anette (författare)
- Malmö University,Malmö universitet,Institutionen för biomedicinsk vetenskap (BMV),Biofilms Research Center for Biointerfaces
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(creator_code:org_t)
- 2021-04-05
- 2021
- Engelska.
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Ingår i: Applied Sciences. - : MDPI. - 2076-3417. ; 11:7
- Relaterad länk:
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https://doi.org/10.3...
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https://umu.diva-por... (primary) (Raw object)
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https://www.mdpi.com...
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https://mau.diva-por... (primary) (Raw object)
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http://dx.doi.org/10... (free)
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https://urn.kb.se/re...
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https://doi.org/10.3...
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https://urn.kb.se/re...
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https://lup.lub.lu.s...
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Abstract
Ämnesord
Stäng
- Sialylations are post-translational modifications of proteins and lipids that play important roles in many cellular events, including cell-cell interactions, proliferation, and migration. Tumor cells express high levels of sialic acid (SA), which are often associated with the increased invasive potential in clinical tumors, correlating with poor prognosis. To overcome the lack of natural SA-receptors, such as antibodies and lectins with high enough specificity and sensitivity, we have used molecularly imprinted polymers (MIPs), or “plastic antibodies”, as nanoprobes. Because high expression of epithelial cell adhesion molecule (EpCAM) in primary tumors is often associated with proliferation and a more aggressive phenotype, the expression of EpCAM and CD44 was initially analyzed. The SA-MIPs were used for the detection of SA on the cell surface of breast cancer cells. Lectins that specifically bind to the a-2,3 SA and a-2,6 SA variants were used for analysis of SA expression, with both flow cytometry and confocal microscopy. Here we show a correlation of EpCAM and SA expression when using the SA-MIPs for detection of SA. We also demonstrate the binding pattern of the SA-MIPs on the breast cancer cell lines using confocal microscopy. Pre-incubation of the SA-MIPs with SA-derivatives as inhibitors could reduce the binding of the SA-MIPs to the tumor cells, indicating the specificity of the SA-MIPs. In conclusion, the SA-MIPs may be a new powerful tool in the diagnostic analysis of breast cancer cells.
Ämnesord
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinska och farmaceutiska grundvetenskaper -- Cell- och molekylärbiologi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Basic Medicine -- Cell and Molecular Biology (hsv//eng)
- NATURVETENSKAP -- Kemi -- Analytisk kemi (hsv//swe)
- NATURAL SCIENCES -- Chemical Sciences -- Analytical Chemistry (hsv//eng)
- MEDICIN OCH HÄLSOVETENSKAP -- Klinisk medicin -- Cancer och onkologi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Clinical Medicine -- Cancer and Oncology (hsv//eng)
Nyckelord
- Breast cancer
- Epithelial cell adhesion molecule
- Molecularly imprinted polymers
- Nanoparticles
- Sialic acid
- Breast cancer
- Epithelial cell adhesion molecule
- Molecularly imprinted polymers
- Nanoparticles
- Sialic acid
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
Hitta via bibliotek
Till lärosätets databas
- Av författaren/redakt...
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El-Schich, Zahra
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Zhang, Yuecheng
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Göransson, Tommy
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Dizeyi, Nishtman
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Persson, Jenny L ...
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Johansson, Emil, ...
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visa fler...
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Caraballo, Remi
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Elofsson, Mikael
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Shinde, Sudhirku ...
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Sellergren, Börj ...
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Gjörloff Wingren ...
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visa färre...
- Om ämnet
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- MEDICIN OCH HÄLSOVETENSKAP
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MEDICIN OCH HÄLS ...
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och Medicinska och f ...
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och Cell och molekyl ...
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- NATURVETENSKAP
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NATURVETENSKAP
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och Kemi
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och Analytisk kemi
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- MEDICIN OCH HÄLSOVETENSKAP
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MEDICIN OCH HÄLS ...
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och Klinisk medicin
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och Cancer och onkol ...
- Artiklar i publikationen
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Applied Sciences
- Av lärosätet
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Umeå universitet
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Malmö universitet
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Lunds universitet