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The myeloid cell bi...
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Ali, HaythamUmeå University,Umeå universitet,Onkologi,Immunologi/immunkemi,Department of Pathology, Faculty of Veterinary Medicine, Zagazig University, Zagazig, Egypt
(författare)
The myeloid cell biomarker EMR1 is ectopically expressed in colon cancer
- Artikel/kapitelEngelska2021
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IOS Press,2021
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LIBRIS-ID:oai:DiVA.org:umu-188165
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https://urn.kb.se/resolve?urn=urn:nbn:se:umu:diva-188165URI
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https://doi.org/10.3233/TUB-200082DOI
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https://lup.lub.lu.se/record/8b5a3dd4-218d-41e9-a6ed-862c669c98b0URI
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Språk:engelska
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Sammanfattning på:engelska
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OBJECTIVE: The microenvironment of colon cancer (CC) is heterogeneous including cells of myeloid lineage affecting tumor growth and metastasis. Two functional subtypes of myeloid cells have been identified; one (M1) is tumor-inhibitory and the other one (M2) is tumor-promoting. Whether the three myeloid markers EMR1, CD206 and CD86 are expressed only in the infiltrating myeloid cells or also in the tumor cells was investigated.METHODS: Expression of the myeloid markers was investigated in CC at the mRNA and protein levels in primary tumors and lymph nodes. mRNA expression was also determined in 5 CC cell lines. Protein expression was investigated by two-color immunofluorescence and consecutive-sections-immune-staining combined with morphometry using specific antibodies for the myeloid cell markers and the epithelial cell markers CEACAM5 and EpCAM.RESULTS: EMR1 and CD86, but not CD206, mRNA levels were significantly higher in CC primary tumors compared to apparently normal colon tissue (P < 0.0001). EMR1 mRNA levels were significantly higher in both hematoxylin-eosin positive (H&E(+)) and H&E(-) lymph nodes of CC patients compared to control nodes (P = 0.03 and P = 0.01, respectively). EMR1 and CD206 mRNAs were expressed in 4/5 and 5/5 CC cell lines, respectively, while CD86 mRNA was not expressed. Immuno-morphometry revealed that about 20% of the tumor cells expressed EMR1 and CD206. Positive cells were tumor cells as revealed by anti-CEACAM5 and anti-EpCAM staining. The number of EMR1, CD206 and CD86 positive cells were significantly increased in CC primary tumors compared to normal colon tissue (P < 0.0001). However, CD206 was also expressed in normal colonocytes. Only EMR1 showed significantly increased numbers of positive tumor cells in H&E(+) nodes compared to H&E(-) nodes (P = 0.001). EMR1 expression in CC tumor cells correlated with CXCL17 expressing tumor cells.CONCLUSION: EMR1, like the chemokine CXCL17, is ectopically expressed in colon cancer possibly in the same cancer cells.
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Ohlsson, LinaUmeå University,Umeå universitet,Immunologi/immunkemi(Swepub:umu)liaohn00
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Lindmark, GudrunLund University,Lunds universitet,Kirurgi,Forskargrupper vid Lunds universitet,Surgery,Lund University Research Groups(Swepub:lu)med-gli
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Hammarström, Marie-LouiseUmeå University,Umeå universitet,Immunologi/immunkemi(Swepub:umu)maha0008
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Hammarström, StenUmeå University,Umeå universitet,Immunologi/immunkemi(Swepub:umu)stha0002
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Sitohy, BaselUmeå University,Umeå universitet,Onkologi,Immunologi/immunkemi(Swepub:umu)balsiy01
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Umeå universitetOnkologi
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Sammanhörande titlar
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Ingår i:Tumor Biology: IOS Press43:1, s. 209-2231010-42831423-0380
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