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Sökning: onr:"swepub:oai:DiVA.org:umu-191147" > The barrier functio...

The barrier functions of crude cervical mucus plugs against HIV-1 infection in the context of cell-free and cell-to-cell transmission

Mhlekude, Baxolele (författare)
University of Cape Town, Department of Surgery, Groote Schuur Hospital, Observatory, South Africa; TWINCORE, Centre for Experimental and Clinical Infection Research; a joint venture between the Hannover Medical School and the Helmholtz Centre for Infection Research, Institute of Experimental Virology, Hannover; Charité - Universitätsmedizin Berlin, Institute of Virology, Charité Campus Mitte; Berlin Institute of Health, Berlin, Germany
Lenman, Annasara (författare)
TWINCORE, Centre for Experimental and Clinical Infection Research; a joint venture between the Hannover Medical School and the Helmholtz Centre for Infection Research, Institute of Experimental Virology, Hannover
Sidoyi, Phikolomzi (författare)
Faculty of Health Sciences, School of Medicine, Walter Sisulu University, Mthatha, South Africa
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Joseph, Jim (författare)
Department of Human Biology, Walter Sisulu University, Mthatha, South Africa
Kruppa, Jochen (författare)
Charité - Universitätsmedizin Berlin, Institut für Biometrie und Klinische Epidemiologie, Charité Campus Mitte, Berlin, Germany
Businge, Charles Bitamazire (författare)
Department of Obstetrics and Gynaecology, Walter Sisulu University/Nelson Mandela Academic Hospital
Mdaka, Mana Lungisa (författare)
Department of Obstetrics and Gynaecology, Walter Sisulu University/Nelson Mandela Academic Hospital
Konietschke, Frank (författare)
Berlin Institute of Health, Berlin, Germany; Charité - Universitätsmedizin Berlin, Institut für Biometrie und Klinische Epidemiologie, Charité Campus Mitte, Berlin, Germany
Pich, Andreas (författare)
Hannover Medical School, Institute of Toxicology, Core Facility Proteomics, Hannover, Germany
Gerold, Gisa, 1979- (författare)
Umeå universitet,Wallenberg centrum för molekylär medicin vid Umeå universitet (WCMM),Avdelningen för virologi,TWINCORE, Centre for Experimental and Clinical Infection Research; a joint venture between the Hannover Medical School and the Helmholtz Centre for Infection Research, Institute of Experimental Virology, Hannover; Department of Biochemistry, University of Veterinary Medicine Hannover, Hanover, Germany
Goffinet, Christine (författare)
TWINCORE, Centre for Experimental and Clinical Infection Research; a joint venture between the Hannover Medical School and the Helmholtz Centre for Infection Research, Institute of Experimental Virology, Hannover; Charité - Universitätsmedizin Berlin, Institute of Virology, Charité Campus Mitte; Berlin Institute of Health, Berlin, Germany
Mall, Anwar Suleman (författare)
University of Cape Town, Department of Surgery, Groote Schuur Hospital, Observatory, South Africa
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 (creator_code:org_t)
Wolters Kluwer, 2021
2021
Engelska.
Ingår i: AIDS. - : Wolters Kluwer. - 0269-9370 .- 1473-5571. ; 35:13, s. 2105-2117
Relaterad länk:
https://urn.kb.se/re...
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https://doi.org/10.1...
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  • Tidskriftsartikel (refereegranskat)
Abstract Ämnesord
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  • OBJECTIVE: The cervical mucus plugs are enriched with proteins of known immunological functions. We aimed to characterize the anti-HIV-1 activity of the cervical mucus plugs against a panel of different HIV-1 strains in the contexts of cell-free and cell-associated virus.DESIGN: A cohort of consenting HIV-1-negative and HIV-1-positive pregnant women in labour was recruited from Mthatha General Hospital in the Eastern Cape province of South Africa, from whom the cervical mucus plugs were collected in 6 M guanidinium chloride with protease inhibitors and transported to our laboratories at -80 °C.METHODS: Samples were centrifuged to remove insoluble material and dialysed before freeze--drying and subjecting them to the cell viability assays. The antiviral activities of the samples were studied using luminometric reporter assays and flow cytometry. Time-of-addition and BlaM-Vpr virus-cell fusion assays were used to pin-point the antiviral mechanisms of the cervical mucus plugs, before proteomic profiling using liquid chromatography-tandem mass spectrometry.RESULTS: The proteinaceous fraction of the cervical mucus plugs exhibited anti-HIV-1 activity with inter-individual variations and some degree of specificity among different HIV-1 strains. Cell-associated HIV-1 was less susceptible to inhibition by the potent samples whenever compared with the cell-free HIV-1. The samples with high antiviral potency exhibited a distinct proteomic profile when compared with the less potent samples.CONCLUSION: The crude cervical mucus plugs exhibit anti-HIV-1 activity, which is defined by a specific proteomic profile.

Ämnesord

MEDICIN OCH HÄLSOVETENSKAP  -- Klinisk medicin -- Infektionsmedicin (hsv//swe)
MEDICAL AND HEALTH SCIENCES  -- Clinical Medicine -- Infectious Medicine (hsv//eng)

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