Sökning: onr:"swepub:oai:DiVA.org:umu-191885" >
Lipoprotein size is...
Lipoprotein size is a main determinant for the rate of hydrolysis by exogenous LPL in human plasma
-
- Kovrov, Oleg (författare)
- Umeå universitet,Fysiologisk kemi
-
- Landfors, Fredrik (författare)
- Umeå universitet,Avdelningen för medicin
-
- Saar-Kovrov, Valeria (författare)
- Umeå universitet,Fysiologisk kemi,Department of Pathology, CARIM School for Cardiovascular Diseases MUMC+, Maastricht University, Maastricht, Netherlands
-
visa fler...
-
- Näslund, Ulf (författare)
- Umeå universitet,Avdelningen för medicin,Heart Centre
-
- Olivecrona, Gunilla (författare)
- Umeå universitet,Fysiologisk kemi
-
visa färre...
-
(creator_code:org_t)
- American Society for Biochemistry and Molecular Biology, 2022
- 2022
- Engelska.
-
Ingår i: Journal of Lipid Research. - : American Society for Biochemistry and Molecular Biology. - 0022-2275 .- 1539-7262. ; 63:1
- Relaterad länk:
-
https://doi.org/10.1...
-
visa fler...
-
https://umu.diva-por... (primary) (Raw object)
-
http://www.jlr.org/a...
-
https://urn.kb.se/re...
-
https://doi.org/10.1...
-
visa färre...
Abstract
Ämnesord
Stäng
- LPL is a key player in plasma triglyceride metabolism. Consequently, LPL is regulated by several proteins during synthesis, folding, secretion, and transport to its site of action at the luminal side of capillaries, as well as during the catalytic reaction. Some proteins are well known, whereas others have been identified but are still not fully understood. We set out to study the effects of the natural variations in the plasma levels of all known LPL regulators on the activity of purified LPL added to samples of fasted plasma taken from 117 individuals. The enzymatic activity was measured at 25°C using isothermal titration calorimetry. This method allows quantification of the ability of an added fixed amount of exogenous LPL to hydrolyze triglyceride-rich lipoproteins in plasma samples by measuring the heat produced. Our results indicate that, under the conditions used, the normal variation in the endogenous levels of apolipoprotein C1, C2, and C3 or the levels of angiopoietinlike proteins 3, 4, and 8 in the fasted plasma samples had no significant effect on the recorded activity of the added LPL. Instead, the key determinant for the LPL activity was a lipid signature strongly correlated to the average size of the VLDL particles. The signature involved not only several lipoprotein and plasma lipid parameters but also apolipoprotein A5 levels. While the measurements cannot fully represent the action of LPL when attached to the capillary wall, our study provides knowledge on the interindividual variation of LPL lipolysis rates in human plasma.
Ämnesord
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinska och farmaceutiska grundvetenskaper -- Cell- och molekylärbiologi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Basic Medicine -- Cell and Molecular Biology (hsv//eng)
- NATURVETENSKAP -- Biologi -- Biokemi och molekylärbiologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Biochemistry and Molecular Biology (hsv//eng)
- MEDICIN OCH HÄLSOVETENSKAP -- Klinisk medicin -- Endokrinologi och diabetes (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Clinical Medicine -- Endocrinology and Diabetes (hsv//eng)
Nyckelord
- Angiopoietin-like proteins
- Apolipoproteins
- Capillaries
- Exogenous LPL
- Isothermal titration calorimetry
- Lipid signature
- Lipidomics
- Plasma triglyceride metabolism
- VLDL particle size
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
Hitta via bibliotek
Till lärosätets databas