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Attenuation of Pseu...
Attenuation of Pseudomonas aeruginosa infection by INP0341, a salicylidene acylhydrazide, in a murine model of keratitis
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- Sharma, Prerana (författare)
- Prof. Brien Holden Eye Research Centre, LV Prasad Eye Institute, Hyderabad, India; Department of Animal Biology, University of Hyderabad, Hyderabad, India
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- Elofsson, Mikael (författare)
- Umeå universitet,Kemiska institutionen
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- Roy, Sanhita (författare)
- Prof. Brien Holden Eye Research Centre, LV Prasad Eye Institute, Hyderabad, India
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Prof Brien Holden Eye Research Centre, LV Prasad Eye Institute, Hyderabad, India; Department of Animal Biology, University of Hyderabad, Hyderabad, India Kemiska institutionen (creator_code:org_t)
- 2020-06-07
- 2020
- Engelska.
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Ingår i: Virulence. - : Taylor & Francis. - 2150-5594 .- 2150-5608. ; 11:1, s. 795-804
- Relaterad länk:
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https://doi.org/10.1...
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https://umu.diva-por... (primary) (Raw object)
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https://www.tandfonl...
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https://urn.kb.se/re...
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https://doi.org/10.1...
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Abstract
Ämnesord
Stäng
- Pseudomonas aeruginosa: is an opportunistic pathogen and a major cause of corneal infections worldwide. The bacterium secretes several toxins through its type III secretion system (T3SS) to subvert host immune responses. In addition, it is armed with intrinsic as well as acquired antibiotic resistance mechanisms that make treatment a significant challenge and new therapeutic interventions are needed. Type III secretion inhibitors have been studied as an alternative or in accompaniment to traditional antibiotics to inhibit virulence of bacteria. In this study, INP0341, a T3SS inhibitor, inhibited cytotoxicity by P. aeruginosa toward human corneal epithelial cells (HCEC) at 100 μM without affecting bacterial growth in the liquid media. An increased expression of antimicrobial peptides and reactive oxygen species generation was also observed in cells exposed to P. aeruginosa in the presence of INP0341. Furthermore, INP0341 efficiently attenuated corneal infection by P. aeruginosa in an experimental model of murine keratitis as evident from corneal opacity, clinical score and bacterial load. Thus, INP0341 appears to be a promising candidate to treat corneal infection caused by P. aeruginosa and can be further considered as an alternative therapeutic intervention.
Ämnesord
- NATURVETENSKAP -- Biologi -- Immunologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Immunology (hsv//eng)
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinska och farmaceutiska grundvetenskaper -- Mikrobiologi inom det medicinska området (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Basic Medicine -- Microbiology in the medical area (hsv//eng)
Nyckelord
- Antimicrobial peptides
- corneal epithelial cells
- INP0341
- Pseudomonas aeruginosa
- reactive oxygen species
- type III secretion system
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
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