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PcoB is a defense o...
PcoB is a defense outer membrane protein that facilitates cellular uptake of copper
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- Li, Ping (författare)
- Lund University,Lunds universitet,Membranproteinstrukturbiologi,Forskargrupper vid Lunds universitet,Membrane Protein Structural Biology,Lund University Research Groups
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- Nayeri, Niloofar (författare)
- Lund University,Lunds universitet,Membranproteinstrukturbiologi,Forskargrupper vid Lunds universitet,Membrane Protein Structural Biology,Lund University Research Groups
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- Górecki, Kamil (författare)
- Lund University,Lunds universitet,Membranproteinstrukturbiologi,Forskargrupper vid Lunds universitet,Membrane Protein Structural Biology,Lund University Research Groups
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- Becares, Eva Ramos (författare)
- Department of Biomedical Sciences, University of Copenhagen, Copenhagen, Denmark
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- Wang, Kaituo (författare)
- Department of Biomedical Sciences, University of Copenhagen, Copenhagen, Denmark
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- Mahato, Dhani Ram (författare)
- Umeå University,Umeå universitet,Kemiska institutionen
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- Andersson, Magnus (författare)
- Umeå University,Umeå universitet,Kemiska institutionen
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- Abeyrathna, Sameera S. (författare)
- Department of Chemistry and Biochemistry, The University of Texas at Dallas, TX, Richardson, United States
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- Lindkvist-Petersson, Karin (författare)
- Lund University,Lunds universitet,Medicinsk strukturbiologi,Forskargrupper vid Lunds universitet,Medical Structural Biology,Lund University Research Groups
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- Meloni, Gabriele (författare)
- Department of Chemistry and Biochemistry, The University of Texas at Dallas, TX, Richardson, United States
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- Missel, Julie Winkel (författare)
- Department of Biomedical Sciences, University of Copenhagen, Copenhagen, Denmark
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- Gourdon, Pontus (författare)
- Lund University,Lunds universitet,Membranproteinstrukturbiologi,Forskargrupper vid Lunds universitet,Membrane Protein Structural Biology,Lund University Research Groups,University of Copenhagen
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(creator_code:org_t)
- 2022-06-21
- 2022
- Engelska.
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Ingår i: Protein Science. - : Wiley. - 0961-8368 .- 1469-896X. ; 31:7
- Relaterad länk:
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https://doi.org/10.1...
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http://dx.doi.org/10... (free)
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https://urn.kb.se/re...
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https://doi.org/10.1...
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https://lup.lub.lu.s...
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Abstract
Ämnesord
Stäng
- Copper (Cu) is one of the most abundant trace metals in all organisms, involved in a plethora of cellular processes. Yet elevated concentrations of the element are harmful, and interestingly prokaryotes are more sensitive for environmental Cu stress than humans. Various transport systems are present to maintain intracellular Cu homeostasis, including the prokaryotic plasmid-encoded multiprotein pco operon, which is generally assigned as a defense mechanism against elevated Cu concentrations. Here we structurally and functionally characterize the outer membrane component of the Pco system, PcoB, recovering a 2.0 Å structure, revealing a classical β-barrel architecture. Unexpectedly, we identify a large opening on the extracellular side, linked to a considerably electronegative funnel that becomes narrower towards the periplasm, defining an ion-conducting pathway as also supported by metal binding quantification via inductively coupled plasma mass spectrometry and molecular dynamics (MD) simulations. However, the structure is partially obstructed towards the periplasmic side, and yet flux is permitted in the presence of a Cu gradient as shown by functional characterization in vitro. Complementary in vivo experiments demonstrate that isolated PcoB confers increased sensitivity towards Cu. Aggregated, our findings indicate that PcoB serves to permit Cu import. Thus, it is possible the Pco system physiologically accumulates Cu in the periplasm as a part of an unorthodox defense mechanism against metal stress. These results point to a previously unrecognized principle of maintaining Cu homeostasis and may as such also assist in the understanding and in efforts towards combatting bacterial infections of Pco-harboring pathogens.
Ämnesord
- NATURVETENSKAP -- Biologi -- Biokemi och molekylärbiologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Biochemistry and Molecular Biology (hsv//eng)
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinsk bioteknologi -- Medicinsk bioteknologi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Medical Biotechnology -- Medical Biotechnology (hsv//eng)
Nyckelord
- gut microbiota
- outer membrane protein structure
- PcoB
- gut microbiota
- outer membrane protein structure
- PcoB
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
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Till lärosätets databas
- Av författaren/redakt...
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Li, Ping
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Nayeri, Niloofar
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Górecki, Kamil
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Becares, Eva Ram ...
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Wang, Kaituo
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Mahato, Dhani Ra ...
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visa fler...
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Andersson, Magnu ...
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Abeyrathna, Same ...
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Lindkvist-Peters ...
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Meloni, Gabriele
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Missel, Julie Wi ...
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Gourdon, Pontus
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- Om ämnet
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- NATURVETENSKAP
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NATURVETENSKAP
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och Biologi
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och Biokemi och mole ...
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- MEDICIN OCH HÄLSOVETENSKAP
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MEDICIN OCH HÄLS ...
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och Medicinsk biotek ...
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och Medicinsk biotek ...
- Artiklar i publikationen
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Protein Science
- Av lärosätet
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Umeå universitet
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Lunds universitet