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Protocol for isolat...
Protocol for isolation of microbiota-derived membrane vesicles from mouse blood and colon
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- Erttmann, Saskia F. (författare)
- Umeå universitet,Umeå Centre for Microbial Research (UCMR),Institutionen för molekylärbiologi (Medicinska fakulteten),Infection Oncology Unit, Institute of Clinical Molecular Biology, Christian-Albrechts University of Kiel, UKSH, Schwanenweg 20, Kiel, Germany
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- Gekara, Nelson O. (författare)
- Stockholms universitet,Institutionen för molekylär biovetenskap, Wenner-Grens institut,University of Freiburg, Germany
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(creator_code:org_t)
- CellPress, 2023
- 2023
- Engelska.
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Ingår i: STAR Protocols. - : CellPress. - 2666-1667. ; 4:1
- Relaterad länk:
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https://doi.org/10.1...
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https://umu.diva-por... (primary) (Raw object)
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https://urn.kb.se/re...
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https://doi.org/10.1...
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Abstract
Ämnesord
Stäng
- Bacterial membrane vesicles have emerged as gadgets allowing remote communication between the microbiota and distal host organs. Here we describe a protocol for enriching vesicles from serum and colon that could widely be adapted for other tissues. We detail pre-clearing of serum or colon fluids using 0.2-μm syringe filters and their concentration by centrifugal filter devices. We also describe vesicle isolation with qEV size exclusion columns and finally the concentration of isolated vesicle fractions for downstream analyses. For complete details on the use and execution of this protocol, please refer to Erttmann et al. (2022).1
Ämnesord
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinska och farmaceutiska grundvetenskaper -- Cell- och molekylärbiologi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Basic Medicine -- Cell and Molecular Biology (hsv//eng)
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinska och farmaceutiska grundvetenskaper -- Mikrobiologi inom det medicinska området (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Basic Medicine -- Microbiology in the medical area (hsv//eng)
- NATURVETENSKAP -- Biologi -- Mikrobiologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Microbiology (hsv//eng)
Nyckelord
- Cell Separation/Fractionation
- Immunology
- Microbiology
- Model Organisms
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
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