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Topological screen ...
Topological screen identifies hundreds of Cp190- and CTCF-dependent Drosophila chromatin insulator elements
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- Kahn, Tatyana G. (författare)
- Umeå universitet,Institutionen för molekylärbiologi (Medicinska fakulteten)
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- Savitsky, Mikhail (författare)
- Umeå universitet,Institutionen för molekylärbiologi (Medicinska fakulteten)
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- Kuong, Chikuan (författare)
- Department of Computer Science, National Chengchi University, Taipei City, Taiwan
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- Jacquer, Caroline (författare)
- Institute of Human Genetics, UMR9002 CNRS, Montpellier, France
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- Cavalli, Giacomo (författare)
- Institute of Human Genetics, UMR9002 CNRS, Montpellier, France
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- Chang, Jia-Ming (författare)
- Department of Computer Science, National Chengchi University, Taipei City, Taiwan
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- Schwartz, Yuri B. (författare)
- Umeå universitet,Institutionen för molekylärbiologi (Medicinska fakulteten)
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(creator_code:org_t)
- American Association for the Advancement of Science (AAAS), 2023
- 2023
- Engelska.
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Ingår i: Science Advances. - : American Association for the Advancement of Science (AAAS). - 2375-2548. ; 9:5
- Relaterad länk:
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https://doi.org/10.1...
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https://umu.diva-por... (primary) (Raw object)
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https://urn.kb.se/re...
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https://doi.org/10.1...
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Abstract
Ämnesord
Stäng
- Drosophila insulators were the first DNA elements found to regulate gene expression by delimiting chromatin contacts. We still do not know how many of them exist and what impact they have on the Drosophila genome folding. Contrary to vertebrates, there is no evidence that fly insulators block cohesin-mediated chromatin loop extrusion. Therefore, their mechanism of action remains uncertain. To bridge these gaps, we mapped chromatin contacts in Drosophila cells lacking the key insulator proteins CTCF and Cp190. With this approach, we found hundreds of insulator elements. Their study indicates that Drosophila insulators play a minor role in the overall genome folding but affect chromatin contacts locally at many loci. Our observations argue that Cp190 promotes cobinding of other insulator proteins and that the model, where Drosophila insulators block chromatin contacts by forming loops, needs revision. Our insulator catalog provides an important resource to study mechanisms of genome folding.
Ämnesord
- NATURVETENSKAP -- Biologi -- Utvecklingsbiologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Developmental Biology (hsv//eng)
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinsk bioteknologi -- Medicinsk bioteknologi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Medical Biotechnology -- Medical Biotechnology (hsv//eng)
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- art (ämneskategori)
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