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  • Sheng, RenwangSchool of Medicine, Southeast University, Nanjing, China; Center for Stem Cell and Regenerative Medicine, Southeast University, Nanjing, China (författare)

Material stiffness in cooperation with macrophage paracrine signals determines the tenogenic differentiation of mesenchymal stem cells

  • Artikel/kapitelEngelska2023

Förlag, utgivningsår, omfång ...

  • John Wiley & Sons,2023
  • electronicrdacarrier

Nummerbeteckningar

  • LIBRIS-ID:oai:DiVA.org:umu-208058
  • https://urn.kb.se/resolve?urn=urn:nbn:se:umu:diva-208058URI
  • https://doi.org/10.1002/advs.202206814DOI

Kompletterande språkuppgifter

  • Språk:engelska
  • Sammanfattning på:engelska

Ingår i deldatabas

Klassifikation

  • Ämneskategori:ref swepub-contenttype
  • Ämneskategori:art swepub-publicationtype

Anmärkningar

  • Stiffness is an important physical property of biomaterials that determines stem cell fate. Guiding stem cell differentiation via stiffness modulation has been considered in tissue engineering. However, the mechanism by which material stiffness regulates stem cell differentiation into the tendon lineage remains controversial. Increasing evidence demonstrates that immune cells interact with implanted biomaterials and regulate stem cell behaviors via paracrine signaling; however, the role of this mechanism in tendon differentiation is not clear. In this study, polydimethylsiloxane (PDMS) substrates with different stiffnesses are developed, and the tenogenic differentiation of mesenchymal stem cells (MSCs) exposed to different stiffnesses and macrophage paracrine signals is investigated. The results reveal that lower stiffnesses facilitates tenogenic differentiation of MSCs, while macrophage paracrine signals at these stiffnesses suppress the differentiation. When exposed to these two stimuli, MSCs still exhibit enhanced tendon differentiation, which is further elucidated by global proteomic analysis. Following subcutaneous implantation in rats for 2 weeks, soft biomaterial induces only low inflammation and promotes tendon-like tissue formation. In conclusion, the study demonstrates that soft, rather than stiff, material has a greater potential to guide tenogenic differentiation of stem cells, which provides comprehensive evidence for optimized bioactive scaffold design in tendon tissue engineering.

Ämnesord och genrebeteckningar

Biuppslag (personer, institutioner, konferenser, titlar ...)

  • Liu, JiaSchool of Medicine, Southeast University, Nanjing, China; Center for Stem Cell and Regenerative Medicine, Southeast University, Nanjing, China (författare)
  • Zhang, WeiSchool of Medicine, Southeast University, Nanjing, China; Center for Stem Cell and Regenerative Medicine, Southeast University, Nanjing, China; Jiangsu Key Laboratory for Biomaterials and Devices, Southeast University, Nanjing, China; China Orthopedic Regenerative Medicine Group (CORMed), Hangzhou, China (författare)
  • Luo, YifanSchool of Medicine, Southeast University, Nanjing, China; Center for Stem Cell and Regenerative Medicine, Southeast University, Nanjing, China (författare)
  • Chen, ZhixuanSchool of Medicine, Southeast University, Nanjing, China; Center for Stem Cell and Regenerative Medicine, Southeast University, Nanjing, China (författare)
  • Chi, JiayuSchool of Medicine, Southeast University, Nanjing, China; Center for Stem Cell and Regenerative Medicine, Southeast University, Nanjing, China (författare)
  • Mo, QingyunSchool of Medicine, Southeast University, Nanjing, China; Center for Stem Cell and Regenerative Medicine, Southeast University, Nanjing, China (författare)
  • Wang, MingyueSchool of Medicine, Southeast University, Nanjing, China; Center for Stem Cell and Regenerative Medicine, Southeast University, Nanjing, China (författare)
  • Sun, YuzhiCenter for Stem Cell and Regenerative Medicine, Southeast University, Nanjing, China; Department of Orthopaedic Surgery, Institute of Digital Medicine, Nanjing First Hospital, Nanjing Medical University, Nanjing, China (författare)
  • Liu, ChuanquanSchool of Medicine, Southeast University, Nanjing, China; Center for Stem Cell and Regenerative Medicine, Southeast University, Nanjing, China (författare)
  • Zhang, YananSchool of Medicine, Southeast University, Nanjing, China; Center for Stem Cell and Regenerative Medicine, Southeast University, Nanjing, China (författare)
  • Zhu, YueSchool of Medicine, Southeast University, Nanjing, China; Center for Stem Cell and Regenerative Medicine, Southeast University, Nanjing, China (författare)
  • Kuang, BaianSchool of Medicine, Southeast University, Nanjing, China; Center for Stem Cell and Regenerative Medicine, Southeast University, Nanjing, China (författare)
  • Yan, ChunguangSchool of Medicine, Southeast University, Nanjing, China (författare)
  • Liu, HaoyangSchool of Medicine, Southeast University, Nanjing, China; Center for Stem Cell and Regenerative Medicine, Southeast University, Nanjing, China (författare)
  • Backman, Ludvig J.Umeå universitet,Anatomi(Swepub:umu)lugban04 (författare)
  • Chen, JialinSchool of Medicine, Southeast University, Nanjing, China; Center for Stem Cell and Regenerative Medicine, Southeast University, Nanjing, China; Jiangsu Key Laboratory for Biomaterials and Devices, Southeast University, Nanjing, China; China Orthopedic Regenerative Medicine Group (CORMed), Hangzhou, China (författare)
  • School of Medicine, Southeast University, Nanjing, China; Center for Stem Cell and Regenerative Medicine, Southeast University, Nanjing, ChinaSchool of Medicine, Southeast University, Nanjing, China; Center for Stem Cell and Regenerative Medicine, Southeast University, Nanjing, China; Jiangsu Key Laboratory for Biomaterials and Devices, Southeast University, Nanjing, China; China Orthopedic Regenerative Medicine Group (CORMed), Hangzhou, China (creator_code:org_t)

Sammanhörande titlar

  • Ingår i:Advanced Science: John Wiley & Sons10:172198-3844

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