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Impairment of the n...
Impairment of the non-catalytic subunit Dpb2 of DNA Pol ɛ results in increased involvement of Pol δ on the leading strand
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- Dmowski, Michal (författare)
- Institute of Biochemistry and Biophysics, Polish Academy of Sciences, Pawinskiego 5A, Warsaw, Poland
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- Makiela-Dzbenska, Karolina (författare)
- Institute of Biochemistry and Biophysics, Polish Academy of Sciences, Pawinskiego 5A, Warsaw, Poland
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- Sharma, Sushma (författare)
- Umeå universitet,Institutionen för medicinsk kemi och biofysik
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- Chabes, Andrei, Professor (författare)
- Umeå universitet,Institutionen för medicinsk kemi och biofysik
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- Fijalkowska, Iwona J. (författare)
- Institute of Biochemistry and Biophysics, Polish Academy of Sciences, Pawinskiego 5A, Warsaw, Poland
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(creator_code:org_t)
- Elsevier, 2023
- 2023
- Engelska.
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Ingår i: DNA Repair. - : Elsevier. - 1568-7864 .- 1568-7856. ; 129
- Relaterad länk:
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https://doi.org/10.1...
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https://umu.diva-por... (primary) (Raw object)
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https://urn.kb.se/re...
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https://doi.org/10.1...
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Abstract
Ämnesord
Stäng
- The generally accepted model assumes that leading strand synthesis is performed by Pol ε, while lagging-strand synthesis is catalyzed by Pol δ. Pol ε has been shown to target the leading strand by interacting with the CMG helicase [Cdc45 Mcm2–7 GINS(Psf1–3, Sld5)]. Proper functioning of the CMG-Pol ɛ, the helicase-polymerase complex is essential for its progression and the fidelity of DNA replication. Dpb2p, the essential non-catalytic subunit of Pol ε plays a key role in maintaining the correct architecture of the replisome by acting as a link between Pol ε and the CMG complex. Using a temperature-sensitive dpb2–100 mutant previously isolated in our laboratory, and a genetic system which takes advantage of a distinct mutational signature of the Pol δ-L612M variant which allows detection of the involvement of Pol δ in the replication of particular DNA strands we show that in yeast cells with an impaired Dpb2 subunit, the contribution of Pol δ to the replication of the leading strand is significantly increased.
Ämnesord
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinska och farmaceutiska grundvetenskaper -- Medicinsk genetik (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Basic Medicine -- Medical Genetics (hsv//eng)
Nyckelord
- CMG (Cdc45 Mcm2–7 GINS)
- DNA polymerase delta
- DNA polymerase epsilon
- DNA replication fidelity
- Dpb2
- Genome stability
- Pol δ
- Pol ε
- Replication fork
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
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