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Experimental phasin...
Experimental phasing opportunities for macromolecular crystallography at very long wavelengths
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- El Omari, Kamel (författare)
- Diamond Light Source, Harwell Science and Innovation Campus, United Kingdom; Research Complex at Harwell, Rutherford Appleton Laboratory, Didcot, United Kingdom
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- Duman, Ramona (författare)
- Diamond Light Source, Harwell Science and Innovation Campus, United Kingdom; Research Complex at Harwell, Rutherford Appleton Laboratory, Didcot, United Kingdom
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- Mykhaylyk, Vitaliy (författare)
- Diamond Light Source, Harwell Science and Innovation Campus, United Kingdom; Research Complex at Harwell, Rutherford Appleton Laboratory, Didcot, United Kingdom
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visa fler...
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- Orr, Christian M. (författare)
- Diamond Light Source, Harwell Science and Innovation Campus, United Kingdom; Research Complex at Harwell, Rutherford Appleton Laboratory, Didcot, United Kingdom
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- Latimer-Smith, Merlyn (författare)
- Diamond Light Source, Harwell Science and Innovation Campus, United Kingdom
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- Winter, Graeme (författare)
- Diamond Light Source, Harwell Science and Innovation Campus, United Kingdom
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- Grama, Vinay (författare)
- Diamond Light Source, Harwell Science and Innovation Campus, United Kingdom
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- Qu, Feng (författare)
- Research Complex at Harwell, Rutherford Appleton Laboratory, Didcot, United Kingdom; Department of Life Sciences, Imperial College London, London, United Kingdom; Department of Biochemistry, University of Oxford, Oxford, United Kingdom
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- Bountra, Kiran (författare)
- Research Complex at Harwell, Rutherford Appleton Laboratory, Didcot, United Kingdom; Department of Life Sciences, Imperial College London, London, United Kingdom
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- Kwong, Hok Sau (författare)
- Research Complex at Harwell, Rutherford Appleton Laboratory, Didcot, United Kingdom; Department of Life Sciences, Imperial College London, London, United Kingdom
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- Romano, Maria (författare)
- Research Complex at Harwell, Rutherford Appleton Laboratory, Didcot, United Kingdom; Department of Life Sciences, Imperial College London, London, United Kingdom; Institute of Biostructures and Bioimaging, IBB, CNR, Naples, Italy; Department of Pharmacy, University of Naples “Federico II”, Naples, Italy
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- Reis, Rosana I. (författare)
- National Physical Laboratory, Hampton Road, Teddington, United Kingdom
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- Vogeley, Lutz (författare)
- Charles River Discovery Research Services UK, Chesterford Research Park, Saffron Walden, United Kingdom; Max Planck Institute of Molecular Cell Biology and Genetics (MPI-CBG), Dresden, Germany
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- Vecchia, Luca (författare)
- Division of Structural Biology, Wellcome Centre for Human Genetics, University of Oxford, Oxford, United Kingdom; Friedrich Miescher Institute for Biomedical Research, Basel, Switzerland
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- Owen, C. David (författare)
- Diamond Light Source, Harwell Science and Innovation Campus, United Kingdom; Research Complex at Harwell, Rutherford Appleton Laboratory, Didcot, United Kingdom
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- Wittmann, Sina (författare)
- Department of Biochemistry, University of Oxford, Oxford, United Kingdom; Institute of Molecular Biology (IMB), Ackermannweg 4, Mainz, Germany
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- Renner, Max (författare)
- Umeå universitet,Kemiska institutionen,Division of Structural Biology, Wellcome Centre for Human Genetics, University of Oxford, Oxford, United Kingdom
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- Senda, Miki (författare)
- Structural Biology Research Center, Institute of Materials Structure Science, High Energy Accelerator Research Organization (KEK), Ibaraki, Tsukuba, Japan
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- Matsugaki, Naohiro (författare)
- Structural Biology Research Center, Institute of Materials Structure Science, High Energy Accelerator Research Organization (KEK), Ibaraki, Tsukuba, Japan; Department of Materials Structure Science, School of High Energy Accelerator Science, The Graduate University of Advanced Studies (Soken-dai), 1-1 Oho, Ibaraki, Tsukuba, Japan
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- Kawano, Yoshiaki (författare)
- Advanced Photon Technology Division, RIKEN SPring-8 Center, Hyogo, Japan
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- Bowden, Thomas A. (författare)
- Division of Structural Biology, Wellcome Centre for Human Genetics, University of Oxford, Oxford, United Kingdom
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- Moraes, Isabel (författare)
- National Physical Laboratory, Hampton Road, Teddington, United Kingdom
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- Grimes, Jonathan M. (författare)
- Division of Structural Biology, Wellcome Centre for Human Genetics, University of Oxford, Oxford, United Kingdom
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- Mancini, Erika J. (författare)
- School of Life Sciences, University of Sussex, Falmer, Brighton, United Kingdom
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- Walsh, Martin A. (författare)
- Diamond Light Source, Harwell Science and Innovation Campus, United Kingdom; Research Complex at Harwell, Rutherford Appleton Laboratory, Didcot, United Kingdom
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- Guzzo, Cristiane R. (författare)
- Department of Microbiology, Institute of Biomedical Sciences, University of São Paulo, São Paulo, Brazil
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- Owens, Raymond J. (författare)
- Division of Structural Biology, Wellcome Centre for Human Genetics, University of Oxford, Oxford, United Kingdom; The Rosalind Franklin Institute, Harwell Campus, Oxford, United Kingdom
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- Jones, E. Yvonne (författare)
- Division of Structural Biology, Wellcome Centre for Human Genetics, University of Oxford, Oxford, United Kingdom
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- Brown, David G. (författare)
- Charles River Discovery Research Services UK, Chesterford Research Park, Saffron Walden, United Kingdom
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- Stuart, Dave I. (författare)
- Diamond Light Source, Harwell Science and Innovation Campus, United Kingdom; Division of Structural Biology, Wellcome Centre for Human Genetics, University of Oxford, Oxford, United Kingdom
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- Beis, Konstantinos (författare)
- Research Complex at Harwell, Rutherford Appleton Laboratory, Didcot, United Kingdom; Department of Life Sciences, Imperial College London, London, United Kingdom
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- Wagner, Armin (författare)
- Diamond Light Source, Harwell Science and Innovation Campus, United Kingdom; Research Complex at Harwell, Rutherford Appleton Laboratory, Didcot, United Kingdom
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(creator_code:org_t)
- Nature Publishing Group, 2023
- 2023
- Engelska.
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Ingår i: Communications Chemistry. - : Nature Publishing Group. - 2399-3669. ; 6:1
- Relaterad länk:
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https://doi.org/10.1...
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https://umu.diva-por... (primary) (Raw object)
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https://urn.kb.se/re...
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https://doi.org/10.1...
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Abstract
Ämnesord
Stäng
- Despite recent advances in cryo-electron microscopy and artificial intelligence-based model predictions, a significant fraction of structure determinations by macromolecular crystallography still requires experimental phasing, usually by means of single-wavelength anomalous diffraction (SAD) techniques. Most synchrotron beamlines provide highly brilliant beams of X-rays of between 0.7 and 2 Å wavelength. Use of longer wavelengths to access the absorption edges of biologically important lighter atoms such as calcium, potassium, chlorine, sulfur and phosphorus for native-SAD phasing is attractive but technically highly challenging. The long-wavelength beamline I23 at Diamond Light Source overcomes these limitations and extends the accessible wavelength range to λ = 5.9 Å. Here we report 22 macromolecular structures solved in this extended wavelength range, using anomalous scattering from a range of elements which demonstrate the routine feasibility of lighter atom phasing. We suggest that, in light of its advantages, long-wavelength crystallography is a compelling option for experimental phasing.
Ämnesord
- NATURVETENSKAP -- Fysik -- Atom- och molekylfysik och optik (hsv//swe)
- NATURAL SCIENCES -- Physical Sciences -- Atom and Molecular Physics and Optics (hsv//eng)
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El Omari, Kamel
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Duman, Ramona
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Mykhaylyk, Vital ...
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Orr, Christian M ...
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Latimer-Smith, M ...
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Winter, Graeme
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visa fler...
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Grama, Vinay
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Qu, Feng
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Bountra, Kiran
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Kwong, Hok Sau
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Romano, Maria
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Reis, Rosana I.
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Vogeley, Lutz
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Vecchia, Luca
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Owen, C. David
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Wittmann, Sina
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Renner, Max
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Senda, Miki
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Matsugaki, Naohi ...
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Kawano, Yoshiaki
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Bowden, Thomas A ...
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Moraes, Isabel
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Grimes, Jonathan ...
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Mancini, Erika J ...
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Walsh, Martin A.
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Guzzo, Cristiane ...
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Owens, Raymond J ...
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Jones, E. Yvonne
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Brown, David G.
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Stuart, Dave I.
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Beis, Konstantin ...
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Wagner, Armin
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