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Hybrid capture-base...
Hybrid capture-based next-generation sequencing of new and old world Orthohantavirus strains and wild-type Puumala isolates from humans and bank voles
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- Rosenbaum, William (författare)
- Umeå universitet,Institutionen för medicinsk biovetenskap
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- Bovinder Ylitalo, Erik (författare)
- Umeå universitet,Institutionen för diagnostik och intervention
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- Castel, Guillaume (författare)
- CBGP, INRAE, CIRAD, Institut Agro, IRD, Univ Montpellier, Montpellier, France
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- Sjödin, Andreas (författare)
- CBRN Security and Defence, Swedish Defence Research Agency - FOI, Umeå, Sweden
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- Larsson, Pär (författare)
- Umeå universitet,Medicinsk och klinisk genetik
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- Wigren Byström, Julia (författare)
- Umeå universitet,Institutionen för klinisk mikrobiologi
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- Forsell, Mattias N. E. (författare)
- Umeå universitet,Institutionen för klinisk mikrobiologi
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- Ahlm, Clas, 1956- (författare)
- Umeå universitet,Institutionen för klinisk mikrobiologi
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- Pettersson, Lisa (författare)
- Umeå universitet,Institutionen för klinisk mikrobiologi
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- Tuiskunen-Bäck, Anne (författare)
- Umeå universitet,Institutionen för klinisk mikrobiologi
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(creator_code:org_t)
- Elsevier, 2024
- 2024
- Engelska.
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Ingår i: Journal of Clinical Virology. - : Elsevier. - 1386-6532 .- 1873-5967. ; 172
- Relaterad länk:
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https://doi.org/10.1...
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https://umu.diva-por... (primary) (Raw object)
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https://urn.kb.se/re...
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https://doi.org/10.1...
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Abstract
Ämnesord
Stäng
- Orthohantaviruses, transmitted primarily by rodents, cause hemorrhagic fever with renal syndrome (HFRS) in Eurasia and hantavirus pulmonary syndrome in the Americas. These viruses, with documented human-to-human transmission, exhibit a wide case-fatality rate, 0.5–40 %, depending on the virus species, and no vaccine or effective treatment for severe Orthohantavirus infections exists. In Europe, the Puumala virus (PUUV), carried by the bank vole Myodes glareolus, causes a milder form of HFRS. Despite the reliance on serology and PCR for diagnosis, the three genomic segments of Swedish wild-type PUUV have yet to be completely sequenced.We have developed a targeted hybrid-capture method aimed at comprehensive genomic sequencing of wild-type PUUV isolates and the identification of other Orthohantaviruses. Our custom-designed panel includes >11,200 probes covering the entire Orthohantavirus genus. Using this panel, we sequenced complete viral genomes from bank vole lung tissue, human plasma samples, and cell-cultured reference strains. Analysis revealed that Swedish PUUV isolates belong to the Northern Scandinavian lineage, with nucleotide diversity ranging from 2.8 % to 3.7 % among them. Notably, no significant genotypic differences were observed between the viral sequences from reservoirs and human cases except in the nonstructural protein.Despite the high endemicity of PUUV in Northern Sweden, these are the first complete Swedish wild-type PUUV genomes and substantially increase our understanding of PUUV evolution and epidemiology. The panel's sensitivity enables genomic sequencing of human samples with viral RNA levels reflecting the natural progression of infection and underscores our panel's diagnostic value, and could help to uncover novel Orthohantavirus transmission routes.
Ämnesord
- MEDICIN OCH HÄLSOVETENSKAP -- Klinisk medicin -- Infektionsmedicin (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Clinical Medicine -- Infectious Medicine (hsv//eng)
Nyckelord
- Targeted sequencing
- Whole-genome sequencing
- Puumala virus
- Orthohantaviruses
- Hemorrhagic fever with renal syndrome
- Diagnostics
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
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Till lärosätets databas
- Av författaren/redakt...
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Rosenbaum, Willi ...
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Bovinder Ylitalo ...
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Castel, Guillaum ...
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Sjödin, Andreas
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Larsson, Pär
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Wigren Byström, ...
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visa fler...
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Forsell, Mattias ...
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Ahlm, Clas, 1956 ...
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Pettersson, Lisa
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Tuiskunen-Bäck, ...
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visa färre...
- Om ämnet
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- MEDICIN OCH HÄLSOVETENSKAP
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MEDICIN OCH HÄLS ...
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och Klinisk medicin
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och Infektionsmedici ...
- Artiklar i publikationen
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Journal of Clini ...
- Av lärosätet
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Umeå universitet